Application of Idarubicin loaded Equalspheres in the TACE procedure for Rabbit Liver Tumor Model.

IF 3.9 2区 医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Huibin Lu, Zeyi Yao, Yahua Li, Xiaoyong Ge, Zongming Li, Zihe Zhou, Yifan Li, Tengfei Jin, Yao Ge, Yangduan Yao, Jianzhuang Ren, Xinwei Han, Kewei Ren
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引用次数: 0

Abstract

Rationale and objectives: To investigate the in vitro drug loading and release effects of uniformly sized beads Equalspheres (ES) for idarubicin (IDA) and its application in rabbit liver VX₂ tumor model.

Material and methods: The ES sizes of 70 µm and 100 µm were divided into three groups according to different IDA and ES ratios and different IDA concentrations for the drug loading experiment. The optimal concentration group was selected for the drug release experiment. The diameter and Zeta potential of the ES was measured, and the shrinkage rate was calculated. The rabbit liver VX₂ tumor model was divided into a control group, a TACE group, and an IDA-TACE group. The HE and IHC staining data were compared and analyzed.

Results: The shrinkage of 70 and 100 µm ES with IDA loaded exceeds 20%. Both 70 µm and 100 µm ES can rapidly load IDA, and ES's loading efficiency with 10mg/ml IDA is higher than that with 5mg/ml. The encapsulation rate of 70 µm ES with IDA concentrations of 10mg/ml and 5mg/ml after 5 min of drug administration were (90.75±3.57)% and (83.97±2.34)%, respectively. The encapsulation rates of 100 µm ES with IDA concentrations of 10mg/ml and 5mg/ml after 5 min of drug administration were (93.66±2.19)% and (89.92±1.52)%, respectively. The 1 h and 24 h release rates of 70 µm ES were (62.64±1.88)% and (88.77±7.27)%, respectively. The 1 h and 24 h release rates of 100 µm ES were (80.74±4.90)% and (96.42±9.48)%, respectively. The 70 µm ES was chosen to perform TACE and IDA-TACE. Pathological results suggested that the expression of PCNA in the tumor after IDA-TACE was significantly downregulated, while the expression of VEGF, CD31, and HIF-1 was increased after embolization in 1w and significantly decreased in 2w.

Conclusion: This study demonstrated that ES can rapidly load IDA and achieve sustained release. The shrinkage of ES needs to be a concern in clinical applications. The IDA-TACE is an effective procedure for achieving hepatic tumor locoregional treatment.

负载依达柔比星的Equalspheres在兔肝肿瘤模型TACE中的应用。
理由与目的:研究大小均匀的Equalspheres (ES)对伊达柔比星(IDA)的体外载释作用及其在兔肝VX₂肿瘤模型中的应用。材料与方法:将粒径为70µm和100µm的ES根据不同的IDA和ES比及不同的IDA浓度分为三组进行载药实验。选择最佳浓度组进行药物释放实验。测量了ES的直径和Zeta电位,并计算了收缩率。将兔肝VX 2肿瘤模型分为对照组、TACE组和IDA-TACE组。对比分析HE和IHC染色数据。结果:加载IDA的70、100µm ES收缩率均超过20%。70µm和100µm ES均能快速加载IDA,且10mg/ml IDA时ES的加载效率高于5mg/ml。IDA浓度为10mg/ml和5mg/ml的70µm ES在给药5 min后的包封率分别为(90.75±3.57)%和(83.97±2.34)%。IDA浓度为10mg/ml和5mg/ml的100µm ES在给药5min后的包封率分别为(93.66±2.19)%和(89.92±1.52)%。70µm ES的1 h和24 h释放率分别为(62.64±1.88)%和(88.77±7.27)%。100µm ES的1 h和24 h释放率分别为(80.74±4.90)%和(96.42±9.48)%。选择70µm ES进行TACE和IDA-TACE。病理结果显示,IDA-TACE栓塞后肿瘤中PCNA表达明显下调,VEGF、CD31、HIF-1表达在栓塞后1w升高,2w显著降低。结论:本研究表明ES能快速加载IDA并实现缓释。ES的收缩在临床应用中需要引起关注。IDA-TACE是实现肝肿瘤局部治疗的有效方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Academic Radiology
Academic Radiology 医学-核医学
CiteScore
7.60
自引率
10.40%
发文量
432
审稿时长
18 days
期刊介绍: Academic Radiology publishes original reports of clinical and laboratory investigations in diagnostic imaging, the diagnostic use of radioactive isotopes, computed tomography, positron emission tomography, magnetic resonance imaging, ultrasound, digital subtraction angiography, image-guided interventions and related techniques. It also includes brief technical reports describing original observations, techniques, and instrumental developments; state-of-the-art reports on clinical issues, new technology and other topics of current medical importance; meta-analyses; scientific studies and opinions on radiologic education; and letters to the Editor.
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