DNA N6-methyladenine and ABCA1 methylation associations with subclinical coronary atherosclerosis in people with HIV.

IF 4.4 2区医学 Q1 GENETICS & HEREDITY

Clinical Epigenetics Pub Date : 2025-10-03 DOI:10.1186/s13148-025-01977-y

Jose I Bernardino, Regina Dalmau, Diego Rodriguez-Torres, Gabriel Saiz-Medrano, María Fernandez-Velilla, Inmaculada Pinilla, Javier Rodriguez-Centeno, Andrés Esteban-Cantos, María Jiménez-González, Tatiana Mata, Carmen Ramos, Berta Rodés, Miriam Estébanez

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引用次数: 0

Abstract

ABCA1 methylation and N⁶-methyladenine DNA have been identified as epigenetic markers in coronary artery disease. We performed a cross-sectional exploratory study to analyze whether global DNA methylation and ABCA1 promoter methylation in monocytes are associated with coronary atherosclerosis in asymptomatic HIV individuals without cardiovascular disease. Coronary atherosclerosis was defined as a coronary plaque in at least one coronary segment. We included 27 individuals, 15 of whom had coronary atherosclerosis. ABCA1 DNA methylation was higher in participants with coronary atherosclerosis, with statistically significant differences at specific loci related to the activator protein-2 (AP-2) binding site. There were no differences in global levels of 5-methylcytosine and N⁶-methyladenine DNA between individuals with or without coronary atherosclerosis. ABCA1 methylation showed moderate correlations with both the Leaman score and coronary plaque volume, whereas global DNA N⁶-methyladenine had a negative moderate correlation with the segment severity score and LDL cholesterol. Among participants with coronary atherosclerosis, we found a strong negative correlation between ABCA1 methylation and total atheroma plaque volume, with certain loci (CpG 9 and 12) exhibiting very strong associations.

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DNA n6 -甲基腺嘌呤和ABCA1甲基化与HIV感染者亚临床冠状动脉粥样硬化的关系

ABCA1甲基化和n6 -甲基腺嘌呤DNA已被确定为冠状动脉疾病的表观遗传标记。我们进行了一项横断面探索性研究，分析单核细胞中DNA甲基化和ABCA1启动子甲基化是否与无心血管疾病的无症状HIV个体的冠状动脉粥样硬化相关。冠状动脉粥样硬化定义为至少一个冠状动脉段出现冠状动脉斑块。我们纳入了27人，其中15人患有冠状动脉粥样硬化。冠状动脉粥样硬化患者的ABCA1 DNA甲基化水平较高，与AP-2结合位点相关的特定位点差异有统计学意义。在有或没有冠状动脉粥样硬化的个体之间，5-甲基胞嘧啶和n6 -甲基腺嘌呤DNA的总体水平没有差异。ABCA1甲基化与Leaman评分和冠状动脉斑块体积呈中等相关性，而总DNA n6 -甲基腺嘌呤与节段严重程度评分和LDL胆固醇呈中等负相关。在冠状动脉粥样硬化的参与者中，我们发现ABCA1甲基化与总动脉粥样斑块体积之间存在很强的负相关，某些位点（CpG 9和12）表现出很强的相关性。

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来源期刊

Clinical Epigenetics ONCOLOGY-

自引率

5.30%

发文量

150

期刊介绍： Clinical Epigenetics, the official journal of the Clinical Epigenetics Society, is an open access, peer-reviewed journal that encompasses all aspects of epigenetic principles and mechanisms in relation to human disease, diagnosis and therapy. Clinical trials and research in disease model organisms are particularly welcome.