Biofilm-disrupting heterojunction microneedles: dual ROS amplification and glucose deprivation for accelerated diabetic wound healing.

IF 13.3 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-08-30 eCollection Date: 2025-01-01 DOI:10.7150/thno.120787
Wenjie You, Feng Xiao, Zichao Cai, Jiaxin Zhao, Zhengyao Zhang, Weikang Hu, Yun Chen, Kwang Leong Choy, Zijian Wang
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引用次数: 0

Abstract

Rationale: Diabetic wound healing process is critically hindered by bacterial infection, bacterial biofilm formation, and persistent hyperglycemia. Biomolecular microneedles represent a promising alternative to conventional therapies such as antibiotics and antibiotic-loaded wound dressings, owing to the advantages like reduced risk of drug resistance and enhanced long-term efficacy. However, the microneedles that fulfill the clinical needs of diabetic wounds have rarely been reported. Methods: A glucose oxidase (GOx)-laden Ti3C2/In2O3 (INTG) heterojunction was engineered as a nano-micro platform for reactive oxygen species (ROS) amplification and glucose deprivation, and subsequently immobilized onto the gelatin methacryloyl (GelMA) microneedle tips to obtain double-layer microneedles (GITG microneedles). Their physiochemical properties and biomedical applications were comprehensively investigated. Results: For INTG heterojunction, the formation of Schottky structure significantly improved the oxygen absorption capacity, facilitated the generation and migration of photogenerated electron-hole pairs, thereby promoting the ROS generation. Besides, under near-infrared (NIR) irradiation, GITG microneedles effectively inhibited bacterial proliferation and survival by generating ROS, thereby preventing the formation of bacterial biofilm. Additionally, GITG microneedles accelerated wound closure and facilitated skin tissue regeneration in a rat model through multiple mechanisms. Conclusion: This study developed an advanced microneedle platform enabling on-demand multimodal treatment, demonstrating significant potential for clinical diabetic wound management.

生物膜破坏异质结微针:双ROS扩增和葡萄糖剥夺加速糖尿病伤口愈合。
理由:糖尿病伤口愈合过程受到细菌感染、细菌生物膜形成和持续高血糖的严重阻碍。生物分子微针具有降低耐药风险和提高长期疗效等优点,是替代抗生素和含抗生素伤口敷料等传统疗法的一种很有前景的选择。然而,满足糖尿病创面临床需要的微针却鲜有报道。方法:设计葡萄糖氧化酶(GOx)负载的Ti3C2/In2O3 (INTG)异质结作为活性氧(ROS)扩增和葡萄糖剥夺的纳米微平台,随后将其固定在明胶甲基丙烯酰(GelMA)微针尖端上,获得双层微针(GITG微针)。对其理化性质和生物医学应用进行了全面研究。结果:对于INTG异质结,Schottky结构的形成显著提高了吸氧能力,促进了光生电子-空穴对的生成和迁移,从而促进了ROS的生成。此外,在近红外(NIR)照射下,GITG微针通过产生ROS有效抑制细菌的增殖和存活,从而阻止细菌生物膜的形成。此外,在大鼠模型中,GITG微针通过多种机制加速伤口愈合并促进皮肤组织再生。结论:本研究开发了一种先进的微针平台,可实现按需多模式治疗,在临床糖尿病伤口管理中具有重要潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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