Temporal shifts and cross-site relationships of oral, gut, and vaginal microbiota during the third trimester of pregnancy

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-09-30 DOI:10.1016/j.ygeno.2025.111123

Lulu Meng , Haishan Xie , Xia Duan , Xinyuan Liang , Xiaomei Tang , Huijuan Luo , Xiaomin Xiao , Zhe Li

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引用次数: 0

Abstract

Background

The maternal microbiome during pregnancy influences maternal and neonatal health, yet its dynamics and cross-site relationships in the third trimester remain unclear.

Methods

Oral, fecal, and vaginal samples were collected from 22 healthy pregnant women and analyzed using 16S rRNA gene sequencing.

Results

As pregnancy progressed, gut microbial richness significantly increased, while vaginal richness significantly declined. Source tracking showed that the majority of microbes originated from their respective niches, although low-level cross-site contributions were also observed. Correlation-based network analysis revealed complex associations among microbial communities across sites. The oral microbiome exhibited distinct relative contributions and network relationships to the gut and vaginal microbiomes. Moreover, some low-abundance genera (relative abundance <1 %) played a critical role in maintaining ecological balance compared to high-abundance genera.

Conclusions

This study demonstrates dynamic, site-specific microbial changes and highlights potential microbial connections across body sites during late pregnancy, offering new ecological insights relevant to maternal–fetal health.

查看原文本刊更多论文

妊娠晚期口腔、肠道和阴道微生物群的时间变化和跨部位关系。

背景：妊娠期间母体微生物组影响孕产妇和新生儿健康，但其在妊娠晚期的动态和跨位点关系尚不清楚。方法：采集22例健康孕妇的口腔、粪便和阴道样本，采用16S rRNA基因测序进行分析。结果：随着妊娠的进展，肠道微生物丰富度显著增加，阴道微生物丰富度显著下降。来源跟踪显示，大多数微生物起源于各自的生态位，尽管也观察到低水平的跨站点贡献。基于相关性的网络分析揭示了不同站点间微生物群落之间的复杂关联。口腔微生物组与肠道和阴道微生物组表现出明显的相对贡献和网络关系。结论：本研究显示了妊娠后期体内微生物的动态、位点特异性变化，并强调了妊娠后期身体各部位之间潜在的微生物联系，为母胎健康提供了新的生态学见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.