Low-Input Assay for Transposase-Accessible Chromatin Identifies Epigenetic Signatures of Liver Group 1 Innate Lymphoid Cells

IF 3.7 3区医学 Q2 IMMUNOLOGY

European Journal of Immunology Pub Date : 2025-10-03 DOI:10.1002/eji.70066

Kevin Schmid, Robin P. Schenk, Gabriela M. Wiedemann

引用次数: 0

Abstract

Assessing chromatin accessibility in rare cell populations within tissue remains a key challenge. To address this, we present a low-input ATAC workflow optimized for liver ILCs. The protocol is validated across cell numbers, Tn5 ratios, and library preparation steps and unveils unique epigenetic features of liver NK cells and ILC1s.

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转座酶可及染色质的低输入试验鉴定肝脏1组先天淋巴样细胞的表观遗传特征。

评估组织内罕见细胞群的染色质可及性仍然是一个关键的挑战。为了解决这个问题，我们提出了一个低输入的ATAC工作流程，优化了肝脏ilc。该方案通过细胞数量，Tn5比率和文库制备步骤进行验证，并揭示了肝脏NK细胞和ILC1s的独特表观遗传特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Immunology 医学-免疫学

CiteScore

8.30

自引率

3.70%

发文量

224

审稿时长

2 months

期刊介绍： The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.