The SpyTag/SpyCatcher System: Precise Regulation of Covalent Conjugation and Expansion of Application Scenarios

IF 3.1 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-10-03 DOI:10.1002/biot.70131

Meng-meng Cai, Zi-fu Ni, Zhong-ke Sun, Xiao-long Li, Yan-li Qi, Le Wang, Chao He, Cheng-wei Li

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引用次数: 0

Abstract

The SpyTag/SpyCatcher system is a modular protein assembly tool. Its core mechanism is the formation of isopeptide bonds, which achieves protein assembly through covalent coupling. This system is characterized by mild reaction conditions, rapid connection and no need for additional reagents, and shows good application potential in fields such as enzyme engineering. Although the system has made progress in application, it still faces challenges such as industrial scale and clinical immunogenicity. This paper systematically reviews the principle of the SpyTag/SpyCatcher system and its development progress. It also analyzes the deficiencies of the system in industrial applications, focuses on elaborating its specific application examples in enzyme engineering, discusses existing challenges, and looks forward to future research directions. Overall, this review aims to provide references and new ideas for research in related fields.

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SpyTag/SpyCatcher系统：共价偶联的精确调控和应用场景的扩展。

SpyTag/SpyCatcher系统是一种模块化蛋白质组装工具。其核心机制是形成异肽键，通过共价偶联实现蛋白质组装。该体系具有反应条件温和、连接速度快、无需额外试剂等特点，在酶工程等领域具有良好的应用潜力。虽然该系统在应用方面取得了进展，但仍面临着工业规模和临床免疫原性等挑战。本文系统地综述了SpyTag/SpyCatcher系统的工作原理及其开发进展。分析了该系统在工业应用中的不足，重点阐述了其在酶工程中的具体应用实例，讨论了存在的挑战，并展望了未来的研究方向。综上所述，本文旨在为相关领域的研究提供参考和新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.