Scaling DNA synthesis with a microchip-based massively parallel synthesis system.

IF 41.7 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2025-10-01 DOI:10.1038/s41587-025-02844-0

Xiandi Zhang,Xiang'er Jiang,Yun Wang,Qinzhen Chen,Hao Jiang,Hu Zhang,Antoni Beltran,Weiya Yang,Tai Chen,Chenglong Liang,Ning Cheng,Yun Huang,Guqiao Ding,Chengwang Xie,Nanfeng Gao,Juntao Liu,Wei Xu,Jinlei Huang,Dong Cai,Lihao Zhu,Songjin Mo,Mengzhe Shen,Wenwei Zhang,Ben Lehner,Ming Ni,Jian Wang,Xun Xu,Yue Shen

引用次数: 0

Abstract

Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an 'identification-sorting-synthesis-recycling' iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.

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基于微芯片的大规模并行合成系统的DNA合成缩放。

目前的高通量DNA合成技术使用复杂的芯片和微流控系统来生产大规模合成的寡核苷酸，但浓度低，对长DNA组装的兼容性有限。在这里，我们报告了一个大规模并行合成系统，其“鉴定-分选-合成-回收”迭代机制应用于高通量DNA合成的微芯片。这种方法将DNA产物浓度提高了4到6个数量级，并简化了大规模基因合成的下游过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

期刊介绍： Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.