{"title":"Plant Biotic Stress: Tools and Techniques for Crop Protection.","authors":"Krishna Ramakrishnan Acharya, Aswin Reddy Chilakala, Muthappa Senthil-Kumar","doi":"10.1007/978-1-0716-4746-2_1","DOIUrl":null,"url":null,"abstract":"<p><p>As climate change continues to impact crop yields, developing strategies to enhance plant tolerance to biotic stress has become increasingly important. This requires a thorough evaluation of the tools and methodologies used to manipulate and study biotic stress tolerance. It is crucial to comprehensively understand both conventional and modern techniques, as well as their effectiveness in addressing the specific needs of the crop under study. Detecting diseases at the early stages of plant development can prevent significant losses in large-scale cultivations. Two broad approaches commonly used to mitigate biotic stresses are eliminating causative agents such as fungi, bacteria, nematodes, viruses, or pests, and imparting resistance to the plant. Although there are similarities in the tools and techniques used to address different biotic stresses, each scenario requires dedicated case studies. It is also essential to stay up to date with the latest developments in plant biotechnology to incorporate a cross-disciplinary approach in conducting and validating experiments. This chapter provides an overview of methods covered in this book ranging from molecular breeding to nondestructive techniques that help achieve the goal of safeguarding plant health.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2966 ","pages":"1-20"},"PeriodicalIF":0.0000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-0716-4746-2_1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
As climate change continues to impact crop yields, developing strategies to enhance plant tolerance to biotic stress has become increasingly important. This requires a thorough evaluation of the tools and methodologies used to manipulate and study biotic stress tolerance. It is crucial to comprehensively understand both conventional and modern techniques, as well as their effectiveness in addressing the specific needs of the crop under study. Detecting diseases at the early stages of plant development can prevent significant losses in large-scale cultivations. Two broad approaches commonly used to mitigate biotic stresses are eliminating causative agents such as fungi, bacteria, nematodes, viruses, or pests, and imparting resistance to the plant. Although there are similarities in the tools and techniques used to address different biotic stresses, each scenario requires dedicated case studies. It is also essential to stay up to date with the latest developments in plant biotechnology to incorporate a cross-disciplinary approach in conducting and validating experiments. This chapter provides an overview of methods covered in this book ranging from molecular breeding to nondestructive techniques that help achieve the goal of safeguarding plant health.
期刊介绍:
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.