Impact of Methamphetamine on the Development of the Forensically Important Species Lucilia sericata (Diptera: Calliphoridae).

IF 0.7 4区医学 Q4 PARASITOLOGY

Journal of Arthropod-Borne Diseases Pub Date : 2024-12-31 eCollection Date: 2024-12-01 DOI:10.18502/jad.v18i4.19341

Seyedeh Zahra Parkhideh, Mehdi Forouzesh, Maryam Akhgari, Kamran Akbarzadeh, Sara Rahimi, Sayena Rafizadeh, Yavar Rassi

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引用次数: 0

Abstract

Background: Drugs or poisons can affect the larvae's developmental period in cadavers, leading to variations in the post-mortem interval (PMI) calculation. One of the most misused psychostimulant drugs in Asia and North America, Methamphetamine (MA), is commonly involved in forensic entomotoxicological situations. This study investigated the impact of various MA concentrations on the developmental rate, morphology, and behavior of Lucilia sericata.

Methods: Lucilia sericata was maintained at 28 °C and 50±10% humidity in the Calliphoridae breeding laboratory at the School of Public Health, Tehran University of Medical Sciences. Chicken liver substrates with varying concentrations of MA (0, 45, 90, and 180 ng/mg) were used to produce L. sericata eggs. Ten samples were randomly selected from each treatment group for morphological analysis, including weight and size measurements, at each sampling stage (every 12 hours up to 300 hours). With a significance level of p< 0.05, the General Linear Model (GLM) Mixed Model ANOVA was used to compare the data.

Results: The development time of L. sericata was shown to be changed by Methamphetamine concentrations, which decreased by 24 hours at 90 and 180 ng/mg. Compared to the control group, larvae and pupae lengths decreased by 1.22 and 0.7 mm, respectively, at 180 ng/mg. Additionally, the weight of the larvae and pupae in the concentration mentioned above decreased by 7.52 and 7 mg, respectively, in comparison to the control group.

Conclusion: The PMI is estimated incorrectly if the presence of Methamphetamine in the corpse is disregarded.

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甲基苯丙胺对法医重要物种绢绿蝇发育的影响（双翅目：绢绿蝇科）。

背景：药物或毒物可影响尸体中幼虫的发育时期，导致死亡后间隔（PMI）计算的变化。甲基苯丙胺（Methamphetamine， MA）是亚洲和北美最常被滥用的精神兴奋剂之一，常被用于法医昆虫毒理学研究。本研究探讨了不同浓度的MA对丝光绿蝇发育速率、形态和行为的影响。方法：德黑兰医科大学公共卫生学院丝光Lucilia sericata孳生实验室28℃、50±10%湿度保存。不同MA浓度（0、45、90和180 ng/mg）的鸡肝基质用于生产丝毛螺杆菌蛋。在每个采样阶段（每12小时至300小时），从每个处理组随机抽取10个样本进行形态学分析，包括体重和尺寸测量。在p< 0.05的显著性水平下，采用一般线性模型（GLM）混合模型方差分析进行数据比较。结果：甲基苯丙胺浓度对丝光l.s icicata的发育时间有影响，在90和180 ng/mg浓度下，丝光l.s icicata的发育时间缩短了24 h。与对照组相比，在180 ng/mg浓度下，幼虫和蛹长度分别缩短1.22和0.7 mm。此外，在上述浓度下，幼虫和蛹的体重分别比对照组降低了7.52和7 mg。结论：如果不考虑尸体中甲基苯丙胺的存在，对PMI的估计是不正确的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

8 weeks

期刊介绍： The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.