Immune modulatory potential of Carica papaya against asbestos-induced acute lung injury through down-regulation of NF-kB and MAPK pathways.

Abstract

Inflammation is the primary response to any sort of injury that provokes an immune system and release of inflammatory mediators. The aim of current study is to explore the anti-inflammatory and therapeutic potential of Carica papaya against inflammation caused by environmental particles i.e. silica, dust, and asbestos in rats. The study included four groups (G1 NC, G2 PC, G3 M.T and G4 H.T) with each having eight male albino rats raised for the period of 14 days. Rats were decapitated on day 14^th and sampling was done for CBC, serum analysis, qTR PCR, and histopathology. Data analysis was done through ANOVA and Tukey's Test. There was significant (P ≤ 0.05) decrease of diffrential leucoctyic cells count in H.T group as compared to PC group. Serum lipid profile showed siginificant (P ≤ 0.05) reduction of choltesrol, triglyceride, and LDL, while enhanced HDL concentration in H.T and M.T groups as comparision to PC group. Heat map was generated for gene expression through qRT PCR which revealed the significant (P ≤ 0.05) up-regulation of IL-1 beta, IL-6, IL-33, IL-17, INF-gamma, TNF alpha, TLR-4, Traf-4 and Traf-6. Whereas IL-4, IL-10, and IGF-1 were being significantly (P ≤ 0.05) down- regulated in PC and increased in M.T and H.T groups. Microscopic examination exhibits distorted parenchyma and destructed walls of alveoli in PC while restoration of parenchyma and alveolar structure was seen in treatment groups. It was concluded that C. papaya has potent anti-inflammatory and mitigate the oxidative stress due to inflammation.