Five-Year Molecular Surveillance Reveals Widespread Foodborne Enteric Viruses in Bivalve Mollusks From Golfo Nuevo, Argentina

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-09-30 DOI:10.1111/1750-3841.70573

Camila Frydman, Samuel Miño, Elena Barbieri, Solange Galeano, Sergio Ramon Vaudagna, Viviana Parreño, Marina Mozgovoj

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Abstract

The present investigation conducted a 5-year longitudinal survey (August 2018–March 2023) of enteric viruses in bivalve mollusks (n = 390) collected from Golfo Nuevo, Puerto Madryn, Argentina. Samples were processed following ISO 15216-1:2017 guidelines. Norovirus genogroups I/II (NoV GI/GII), rotavirus A (RVA), hepatitis A (HAV) and E (HEV) viruses, and adenovirus (AdV) were detected and quantified by RT-qPCR/qPCR, genotyped, and subjected to phylogenetic analysis. At least one virus was detected in 40.7% of composite samples. AdV exhibited the highest prevalence (56.6%), while NoV GII and RVA were detected in 12.4% of samples; HEV and HAV were detected in 5.3% and 4.4%, respectively, and NoV GI was not detected. Mean viral loads ranged from 4.8 to 6.3 log10 genomic copies/g of bivalve mollusks. Temporal trends revealed a significant decline in detection post-pandemic for RVA, NoV GII, and HAV, while AdV and HEV rates remained stable. Genotyping identified pandemic variant NoV GII.4[P16], RVA G8P[1], HAV IA, and AdV A31. These results provide the first comprehensive baseline of enteric virus contamination in Patagonian shellfish. Routine virus monitoring and evidence-based control measures are therefore imperative to safeguard public health and to align Argentine bivalve mollusks with international safety standards.

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五年分子监测显示阿根廷新海湾双壳类软体动物中广泛存在食源性肠道病毒。

本研究对从阿根廷马德林港新海湾采集的双壳类软体动物（n = 390）肠道病毒进行了为期5年的纵向调查（2018年8月至2023年3月）。样品按照ISO 15216-1:2017指南进行处理。采用RT-qPCR/qPCR技术对诺如病毒I/II基因群（NoV GI/GII）、轮状病毒A （RVA）、甲型肝炎病毒（HAV）和戊型肝炎病毒（HEV）、腺病毒（AdV）进行检测和定量，并进行基因分型和系统发育分析。在40.7%的复合样本中至少检测到一种病毒。AdV的患病率最高（56.6%），而NoV GII和RVA在12.4%的样本中检测到；HEV和HAV检出率分别为5.3%和4.4%，未检出NoV GI。双壳类软体动物的平均病毒载量为4.8至6.3 log10基因组拷贝/g。时间趋势显示，大流行后RVA、NoV GII和甲型肝炎的检出率显著下降，而AdV和HEV率保持稳定。基因分型鉴定出大流行变异NoV GII.4[P16]、RVA G8P[1]、HAV IA和AdV A31。这些结果提供了巴塔哥尼亚贝类肠道病毒污染的第一个综合基线。因此，必须采取常规病毒监测和循证控制措施，以保障公众健康，并使阿根廷双壳类软体动物符合国际安全标准。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.