Use of a Dihydroxyacetone Derivative as Protecting Reagent to Phosphorylate Oligonucleotides

IF 2.2

Current protocols Pub Date : 2025-10-01 DOI:10.1002/cpz1.70215

Rémy Lartia

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Abstract

We present here a new reagent enabling the supported synthesis of oligodeoxynucleotides (ODNs) and oligoribonucleotides (ORNs) containing a phosphate group at the 5′-terminal position after complete deprotection. This reagent, derived from a dihydroxyacetone core, contains a dimethoxytrityl (DMTr) group. The procedure for final deprotection is very similar to that routinely used in the synthesis of unmodified ODNs or ORNs. In particular, it preserves the advantages of the “trityl-on” method, namely facile purification by reverse-phase high-performance liquid chromatography (RP-HPLC), short treatment with an acetic acid solution in water, the possibility of on-resin monitoring by trityl cation assay, and the use of equipment commonly founded in chemical laboratories. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.

Basic Protocol: Phosphorylation of oligodeoxyribonucleotides (ODNs)

Alternate Protocol: Phosphorylation of oligoribonucleotides (ORNs)

Support Protocol: Preparation of phosphorylation reagent 1

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用二羟基丙酮衍生物作为保护试剂磷酸化寡核苷酸

我们在这里提出了一种新的试剂，能够在完全脱保护后在5 '端位置合成含有磷酸基的寡脱氧核苷酸（ODNs）和寡核糖核苷酸（orn）。该试剂由二羟基丙酮核心衍生而来，含有二甲氧基三硝基（DMTr）基团。最终脱保护的程序非常类似于合成未改性odn或orn时常规使用的程序。特别是，它保留了“三烷基上”法的优点，即通过反相高效液相色谱法（RP-HPLC）易于纯化，在水中用醋酸溶液进行短时间处理，可以通过三烷基阳离子测定法进行树脂上监测，并使用化学实验室常用的设备。©2025作者。Wiley期刊有限责任公司发表的当前协议：基本协议：寡脱氧核糖核苷酸（ODNs）的磷酸化替代协议：寡核糖核苷酸（orn）的磷酸化支持协议：磷酸化试剂的制备1

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来源期刊

Current protocols

CiteScore

4.00

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0.00%

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