Lipidomics-Guided Ultrasound-Assisted Ethanol Extraction of Avocado Pulp and Evaluation of Bioactive Properties

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-09-30 DOI:10.1111/1750-3841.70550

Bruna B. Neves, Rita Pais, Joana Batista, Marisa Pinho, Tatiana Maurício, Stefano Bonciarelli, Laura Goracci, Bruno Neves, Pedro Domingues, M. Rosário Domingues, Tânia Melo

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引用次数: 0

Abstract

Avocados are a valuable source of lipids with potential applications in food, nutraceutical, and cosmetic products, all requiring food-grade and environmentally friendly extraction methods. Though often enjoyed fresh, avocado pulp is also processed for oil. However, conventional extraction techniques can compromise pulp lipid composition, triggering the need for alternative methods that preserve pulp lipid integrity and meet industrial sustainability standards. Ultrasound probe-assisted extraction using ethanol, a green solvent, could be a sustainable alternative to extract lipids from avocado pulp. In the present study, lipid profiles from Hass Portuguese avocado pulp were characterized using C18 reversed-phase HPLC-MS/MS, and their biological activities were evaluated. Folch-like extraction was employed as a benchmark for comparison. Untargeted lipidomics profiling revealed over 100 lipid species across polar and neutral classes, with triacylglycerides enriched in essential polyunsaturated fatty acids. Ultrasound-assisted ethanol extraction achieved ≈70% lipid purity and yielded a lipid composition comparable to that of the conventional method. Notably, green pulp extract exhibited significant bioactivity, including moderate antioxidant activity and powerful anti-inflammatory effects, inhibiting COX-2 and reducing nitric oxide levels at low concentrations. These findings support the use of ultrasound-assisted ethanol extraction as an efficient and sustainable strategy for obtaining bioactive compounds from avocado pulp, reinforcing its potential incorporation into high-value or even new formulations.

Practical Applications

This study shows an eco-friendly method to extract healthy lipids from Hass avocado pulp using ultrasound-assisted ethanol extraction technique. This method is greener than traditional ones and produces extracts with antioxidant and anti-inflammatory benefits. These natural extracts can be used food, cosmetic, and nutraceutical or supplement industries as natural ingredients applications.

Abstract Image

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脂质组学引导超声辅助乙醇提取牛油果果肉及其生物活性评价。

牛油果是一种有价值的脂质来源，在食品、营养保健品和化妆品中有潜在的应用，所有这些都需要食品级和环保的提取方法。鳄梨果肉通常是新鲜的，但也会加工成油。然而，传统的提取技术可能会损害纸浆的脂质成分，从而引发了对保持纸浆脂质完整性和满足工业可持续性标准的替代方法的需求。超声波探针辅助提取乙醇是一种绿色溶剂，是一种可持续的提取鳄梨果肉脂质的替代方法。本研究采用C18反相高效液相色谱-质谱联用技术对哈斯葡萄牙鳄梨果肉的脂质谱进行了表征，并对其生物活性进行了评价。以折叶样提取为基准进行比较。非靶向脂质组学分析显示，极性和中性类超过100种脂质，三酰甘油富含必需的多不饱和脂肪酸。超声辅助乙醇提取获得了≈70%的脂质纯度，并产生了与常规方法相当的脂质组成。值得注意的是，绿浆提取物表现出显著的生物活性，包括适度的抗氧化活性和强大的抗炎作用，在低浓度下抑制COX-2和降低一氧化氮水平。这些发现支持使用超声波辅助乙醇提取从鳄梨果肉中获得生物活性化合物的有效和可持续的策略，加强了将其纳入高价值甚至新配方的潜力。实际应用：本研究展示了一种利用超声波辅助乙醇提取技术从哈斯鳄梨果肉中提取健康脂质的环保方法。这种方法比传统的方法更环保，并产生具有抗氧化和抗炎作用的提取物。这些天然提取物可用于食品、化妆品和营养保健品或补充剂行业作为天然成分的应用。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.