Integrative transcriptome and metabolome evaluation of melanin biosynthesis in Phyllostachys nigra during low-temperature growth.

Abstract

Plant melanin is an organic molecule commonly used in medicine, food, and chemical industries. However, the molecular underpinnings of plant melanin's biosynthesis and its regulation are still unclear. Phyllostachys nigra is well known for its ornamental value because of its black culms. The black pigments enriched in the epidermis and cortex of P. nigra were identified to be melanin by analyses of its physical and chemical properties. Moreover, the biosynthesis of melanin was examined using comprehensive transcriptomic and metabolomic analyses in P. nigra when grown at low temperatures. Nontargeted metabolite profiling revealed that some indoles, including serotonin, 3-indoleacetic acid, and 1H-indole-3-acetamide, were significantly enriched in P. nigra when grown at low temperatures. Parallel transcriptomic analysis showed that a set of structural genes involved in serotonin biosynthesis was significantly upregulated by low temperatures. By integrating the transcriptome data and weighted gene co-expression network analysis, the essential transcription factors that putatively regulate the biosynthesis of serotonins were revealed. Among those, PnWRKY19-3 was functionally tested and shown to increase the serotonin content in transgenic rice by upregulating OsT5H under low temperature conditions. These findings suggest that PnWRKY19-3 may play a positive role in promoting melanin formation in the culms of P. nigra. According to the two functional genomic platforms, it appears that low temperature stimulates melanin formation in P. nigra by inducing the biosynthesis of indoles. Our research provides new insights into melanin biosynthesis in bamboo, which may be vital to other plant species.