Isograft Obtained From ZDF (Obese Fa/Fa) Rats Does Not Impair Nerve Regeneration Compared to Healthy Isograft.

IF 3.1 3区医学 Q2 CLINICAL NEUROLOGY

Muscle & Nerve Pub Date : 2025-09-29 DOI:10.1002/mus.70028

Mackenzie Grasso, Moe Al-Tekreeti, Ananya Gomatam, Elizabeth Zielinski, Geetanjali Bendale, Jonathan Isaacs

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引用次数: 0

Abstract

Introduction/aims: Both Nerve Autograft and Processed Nerve Allograft (PNA) are Acceptable Options for Reconstructing Nerve Defects. While Small Animal Models Suggest That Autograft Has Superior Neuro-Regenerative Properties, This Advantage Has Not Been Demonstrated in Clinical Outcomes. We Believe That in Some Cases Human Autograft May Be Adversely Affected by Pathologic States Such as Diabetes. We Sought an Appropriate Small Animal Model to Test This Theory.

Methods: 10 male ZDF rats (Obese Fa/Fa) genetically predisposed to have type 2 diabetes were used as donors. 10 male ZDF rats (Lean +/?) were utilized as a healthy control. Sciatic nerves harvested bilaterally from the compromised and control groups were utilized to reconstruct a 15 mm defect in 40 ZDF rats (Lean +/?). At 16 weeks, motor testing and nerve histology were performed.

Results: There was no statistically significant difference in axonal counts between the diabetic-derived (6031 ± 3848) and healthy-derived control groups (5813 ± 2535). The average twitch force in the diabetic donor group reached 82% ± 33% of the twitch force of the normal contralateral limb, compared to 67% ± 28% in the healthy graft group. Tetanic force in the diabetic donor group reached 89% ± 30% normalized force compared to 74% ± 29% for the healthy control graft group. Muscle mass and girth ratio were 78% ± 4% and 79% ± 12% for the diabetic donor group, compared to 74% ± 18% and 78% ± 11% for the healthy group.

Discussion: Isograft obtained from a diabetic donor rat did not demonstrate inferior nerve regeneration compared with "normal" healthy isograft.

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与健康同种异体移植相比，肥胖Fa/Fa大鼠的同种异体移植不损害神经再生。

前言/目的：自体神经移植和同种加工神经移植（PNA）都是神经缺损重建可接受的选择。虽然小动物模型表明自体移植物具有优越的神经再生特性，但这种优势尚未在临床结果中得到证实。我们认为，在某些情况下，人类自体移植物可能会受到病理状态（如糖尿病）的不利影响。我们寻找了一个合适的小动物模型来验证这一理论。方法：以10只2型糖尿病遗传易感雄性ZDF大鼠（肥胖Fa/Fa）为供体。选取10只雄性ZDF大鼠（Lean +/?）作为健康对照。利用40只ZDF大鼠（Lean +/?）的双侧坐骨神经重建受损组和对照组的15 mm缺损。16周时，进行运动测试和神经组织学检查。结果：糖尿病源性对照组（6031±3848）与健康源性对照组（5813±2535）的轴突计数差异无统计学意义。糖尿病供体组的平均肌力为正常对侧肢体肌力的82%±33%，而健康移植物组为67%±28%。糖尿病供体组的破伤风力达到89%±30%，而健康对照组为74%±29%。糖尿病供体组肌肉质量和周长比分别为78%±4%和79%±12%，而健康组为74%±18%和78%±11%。讨论：与“正常”健康的同种异体移植物相比，从糖尿病供体大鼠获得的同种异体移植物没有显示出下神经再生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Muscle & Nerve 医学-临床神经学

CiteScore

6.40

自引率

5.90%

发文量

287

审稿时长

3-6 weeks

期刊介绍： Muscle & Nerve is an international and interdisciplinary publication of original contributions, in both health and disease, concerning studies of the muscle, the neuromuscular junction, the peripheral motor, sensory and autonomic neurons, and the central nervous system where the behavior of the peripheral nervous system is clarified. Appearing monthly, Muscle & Nerve publishes clinical studies and clinically relevant research reports in the fields of anatomy, biochemistry, cell biology, electrophysiology and electrodiagnosis, epidemiology, genetics, immunology, pathology, pharmacology, physiology, toxicology, and virology. The Journal welcomes articles and reports on basic clinical electrophysiology and electrodiagnosis. We expedite some papers dealing with timely topics to keep up with the fast-moving pace of science, based on the referees'' recommendation.