Refeeding with different levels of dietary carbohydrate modulates epigenetic stability through global DNA (de)methylation and histone modifications in juvenile and adult Nile tilapia (Oreochromis niloticus).
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引用次数: 0
Abstract
The Nile tilapia (Oreochromis niloticus) exhibits a strong metabolic response to dietary carbohydrates (CHO). Short-term refeeding after fasting with a high-carbohydrate (HC) diet has been shown to modulate CHO metabolism, but the role of epigenetic regulation in this response remains unclear. This study investigated how short-term refeeding with either a HC [639.2 g kg-1 diet]/low-protein [164.9 g kg-1 diet] (HC/LP) diet or a low-CHO [47.4 g kg-1 diet]/high-protein [607.9 g kg-1 diet] (LC/HP) diet influences global DNA methylation and demethylation, histone modifications, and mRNA levels of epigenetic regulators in the liver and muscle of juvenile and adult Nile tilapia. Following a 4-day fasting period, fish were refed for 4 days with either HC/LP or LC/HP diets. Compared to the fasted state, refeeding with either diet altered epigenetic markers by: (1) decreasing hepatic global DNA 5-mC oxidative derivatives-5-hmdC in juveniles, and both 5-hmdC and 5-cadC in adults; (2) inducing histone hypermethylation and/or hyperacetylation - H3K9ac (hepatic) and H3K36me3 (muscular) in juveniles, and H3K9me3 and H3K9ac (muscular) in adults; and (3) promoting expression of enzymes related to DNA hypermethylation (upregulated dnmt, downregulated tet) and histone hypermethylation/acetylation (upregulated setd1b, kmt2, suv39h1b; downregulated kdm4, sirt5). Diet-specific effects included hepatic H3K36 hypomethylation and H3K9 hypoacetylation in juveniles fed HC/LP, accompanied by upregulation of kdm4b, kdm4c, and sirt5. In adults, HC/LP refeeding induced muscular DNA hypomethylation and H3K9 hypoacetylation, associated with upregulation of tet, sirt2, and sirt5. Refeeding following fasting induced histone hypermethylation and/or hyperacetylation, while HC refeeding was particularly associated with muscular global DNA hypomethylation and histone hypoacetylation/methylation.
尼罗罗非鱼（Oreochromis niloticus）对膳食碳水化合物（CHO）表现出强烈的代谢反应。高碳水化合物（HC）饮食禁食后的短期再喂养已被证明可以调节CHO代谢，但表观遗传调控在这一反应中的作用尚不清楚。本研究研究了短期复饲HC [639.2 g kg-1日粮]/低蛋白[164.9 g kg-1日粮]（HC/LP）日粮或低cho [47.4 g kg-1日粮]/高蛋白[607.9 g kg-1日粮]（LC/HP）日粮对尼罗罗非鱼幼鱼和成鱼肝脏和肌肉中DNA甲基化和去甲基化、组蛋白修饰和表观遗传调控因子mRNA水平的影响。禁食4 d后，分别饲喂HC/LP或LC/HP饲料4 d。与禁食状态相比，再饲喂两种饲料都会改变表观遗传标记：(1)幼鱼的肝脏总DNA 5-mC氧化衍生物-5- hdc降低，成鱼的5- hdc和5-cadC均降低；(2)诱导组蛋白超甲基化和/或超乙酰化——少年组蛋白H3K9ac（肝脏组）和H3K36me3（肌肉组），成人组蛋白H3K9me3和H3K9ac（肌肉组）；(3)促进DNA高甲基化（dnmt上调，tet下调）和组蛋白高甲基化/乙酰化（setd1b、kmt2、suv39h1b上调，kdm4、sirt5下调）相关酶的表达。饮食特异性影响包括HC/LP喂养的幼鱼肝脏H3K36低甲基化和H3K9低乙酰化，并伴有kdm4b、kdm4c和sirt5的上调。在成人中，HC/LP再喂养诱导肌肉DNA低甲基化和H3K9低乙酰化，与tet、sirt2和sirt5上调相关。禁食后再饲喂诱导组蛋白高甲基化和/或高乙酰化，而HC再饲喂与肌肉整体DNA低甲基化和组蛋白低乙酰化/甲基化特别相关。
期刊介绍：
Epigenetics publishes peer-reviewed original research and review articles that provide an unprecedented forum where epigenetic mechanisms and their role in diverse biological processes can be revealed, shared, and discussed.
Epigenetics research studies heritable changes in gene expression caused by mechanisms others than the modification of the DNA sequence. Epigenetics therefore plays critical roles in a variety of biological systems, diseases, and disciplines. Topics of interest include (but are not limited to):
DNA methylation
Nucleosome positioning and modification
Gene silencing
Imprinting
Nuclear reprogramming
Chromatin remodeling
Non-coding RNA
Non-histone chromosomal elements
Dosage compensation
Nuclear organization
Epigenetic therapy and diagnostics
Nutrition and environmental epigenetics
Cancer epigenetics
Neuroepigenetics
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