Intrinsically Fluorescent Nano-Scaled Peptide Aggregates Upon Arginine to Citrulline Swap.

IF 3.6 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2025-09-29 DOI:10.1002/bit.70073

Nauman Nazeer,Anupama Ghimire,Jan K Rainey,William D Lubell,Brian D Wagner,Marya Ahmed

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引用次数: 0

Abstract

The supramolecular assembly of short peptides into ordered structures offers promise for developing bio-nanomaterials with diverse applications in drug delivery, electronics, and optical engineering. Intrinsic fluorescence of polypeptide aggregates is typically associated with delocalization of electron densities in dense hydrogen bonding networks, dipolar coupling of aromatic amino acid residues, and possibly by the 'cluster-derived luminescence' associated with supramolecular structures. In a handful of examples, self-assembly of short peptides has provided ordered intrinsically fluorescent nanostructures. In this study, replacement of a single arginine residue with citrulline in a macrocyclic peptide has led to intrinsic fluorescence. The parent arginine-containing peptide macrocycle was previously shown to adopt a β-sheet conformation that aggregated into nonfluorescent spherical particles. The change from the electrostatic positive charge of the guanidine side chain to a hydrogen bonding neutral urea caused the β-sheet peptide to aggregate into larger-sized intrinsically fluorescent rods by a phenomenon that is ascribed to electron delocalization through π-π stacking interactions.

查看原文本刊更多论文

本质荧光纳米级肽聚集体精氨酸到瓜氨酸交换。

短肽的超分子组装成有序结构为开发生物纳米材料提供了希望，这些材料在药物输送、电子和光学工程中具有多种应用。多肽聚集体的固有荧光通常与致密氢键网络中电子密度的离域、芳香氨基酸残基的偶极偶联以及与超分子结构相关的“团簇衍生发光”有关。在少数例子中，短肽的自组装提供了有序的内在荧光纳米结构。在这项研究中，用大环肽中的瓜氨酸取代单个精氨酸残基导致了内在荧光。母体含精氨酸肽大环先前被证明采用β-片状构象，聚集成非荧光球形颗粒。从胍侧链的静电正电荷到氢键中性尿素的变化导致β-片肽通过π-π堆叠相互作用的电子离域现象聚集成更大尺寸的本质荧光棒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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