Rosa Paola Radice, Giuseppe Martelli, Mauro D'Amora, Pierpaolo Dambruoso, Domenico Paparella, Raffaele Mandarano, Giuseppe Olivo, Massimo Scolaro, Domenico Sarubbi, Alessandro Strumia, Maria Calabrese, Andrea Scapigliati, Francesco Greco, Mary Nardi, Stefano Beccaria, Andrea Costamagna, Luca Brazzi, Domenico Abelardo, Pasquale Raimondo, Gianluca Paternoster
{"title":"Gene expression kinetics in Sepsis After Cardiac Surgery (SACS): a multicentric prospective observational study.","authors":"Rosa Paola Radice, Giuseppe Martelli, Mauro D'Amora, Pierpaolo Dambruoso, Domenico Paparella, Raffaele Mandarano, Giuseppe Olivo, Massimo Scolaro, Domenico Sarubbi, Alessandro Strumia, Maria Calabrese, Andrea Scapigliati, Francesco Greco, Mary Nardi, Stefano Beccaria, Andrea Costamagna, Luca Brazzi, Domenico Abelardo, Pasquale Raimondo, Gianluca Paternoster","doi":"10.1186/s44158-025-00277-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Surviving Sepsis Campaign (SSC) defined Sepsis as \"life-threatening organ dysfunction caused by a dysregulated host response to infection\" (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17-65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352-6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71-6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202).</p><p><strong>Design: </strong>This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis.</p><p><strong>Setting: </strong>Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata.</p><p><strong>Participants: </strong>One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study.</p><p><strong>Interventions: </strong>From each patient 4 blood samples were collected during and at the end of the surgery, following the study design.</p><p><strong>Measurements and main results: </strong>We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups.</p><p><strong>Conclusion: </strong>These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.</p>","PeriodicalId":73597,"journal":{"name":"Journal of Anesthesia, Analgesia and Critical Care (Online)","volume":"5 1","pages":"57"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12465282/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Anesthesia, Analgesia and Critical Care (Online)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s44158-025-00277-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: Surviving Sepsis Campaign (SSC) defined Sepsis as "life-threatening organ dysfunction caused by a dysregulated host response to infection" (De Backer D et al, Crit Care Med, n.d.). Sepsis remains one of the leading causes of morbidity and mortality (17-65% (De Oliveira DC, Arq Bras Cardiol Sociedade Brasileira de Cardiologia - SBC 94:352-6, 2010)) worldwide and it still remains a challenge to be defined and for which an appropriate treatment is desired (Chiu and Legrand, Curr Opin Anaesthesiol 34:71-6, 2021). Different studies have been conducted on genes coding for inflammatory cytokines whose could predispose to the development of sepsis [e.g., IL-10 PD1 and WT1] (Gupta DL et al, Infectious Process Sepsis, 202).
Design: This multicentric observational prospective study aims to evaluate blinding the genetic expression kinetics of different molecules involved in the inflammatory process, IL10, PD1 and WT1, to search for a possible molecular predictive marker of sepsis.
Setting: Nine University teaching Hospitals in Italy take part in this study in collaboration with the Department of Applied Science (DISBA) of the University of Basilicata.
Participants: One hundred sixty-two patients, under elective cardiac and on pump surgery were enrolled in the study.
Interventions: From each patient 4 blood samples were collected during and at the end of the surgery, following the study design.
Measurements and main results: We observed, 30 min after the start of the surgery, lower gene expression levels of IL10 and PD1 in septic patients compared to non-septic (p < 0.05), but considering all the timepoint there are differences in gene expression modulation between the groups.
Conclusion: These results confirmed the dysregulated immune response in septic patients compared to non-septic, highlight how a measurement of the gene expression could help to optimize procedures and pay attention to more susceptible patients.
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