A novel self-inhaled nicotine exposure system in murine models reveals age-dependent metabolic effects of nicotine.

IF 6.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell and Bioscience Pub Date : 2025-09-26 DOI:10.1186/s13578-025-01473-z

Xu Xu, Hailan Liu, Meng Yu, Qingzhuo Liu, Yongxiang Li, Yue Deng, Yuxue Yang, Jingjing Cheng, Yutian Liu, Mengjie Wang, Xing Fang, Longlong Tu, Olivia Z Ginnard, Kristine M McDermott, Darah Threat, Fuhui Wang, Meixin Sun, Xinming Liu, Jiamin Qiu, Xi Wu, Jinjing Jian, Tong Zhou, Megan E Burt, Sanika V Jossy, Junying Han, Chunmei Wang, Yongjie Yang, Yong Xu, Yuhan Shi

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引用次数: 0

Abstract

Background: Nicotine is known to influence metabolism and body weight, yet existing murine models of nicotine exposure often fail to replicate human smoking behaviors accurately. To address this gap, we designed a cost-effective, self-administered nicotine inhalation system that allows mice to voluntarily inhale nicotine vapor generated from e-liquid.

Results: Using this system, we investigated the metabolic effects of nicotine exposure in adult and adolescent mice. Adult mice exposed to nicotine vapor for 10 days exhibited significant weight loss, which was rapidly regained upon cessation, paralleling human smoking patterns. In contrast, adolescent mice did not lose weight during nicotine exposure but experienced increased fat mass accumulation after cessation. These results highlight critical age-dependent differences in nicotine's metabolic effects and challenge the common perception that nicotine aids in weight control among adolescents.

Conclusions: Our novel e-liquid-based inhalation model provides a valuable tool for studying nicotine's physiological and behavioral effects, with implications for understanding smoking-related health risks and addiction mechanisms.

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一种新的自吸入尼古丁暴露系统在小鼠模型中揭示了尼古丁的年龄依赖性代谢效应。

背景：已知尼古丁会影响新陈代谢和体重，但现有的尼古丁暴露小鼠模型往往不能准确地复制人类吸烟行为。为了解决这一差距，我们设计了一种具有成本效益的、自我管理的尼古丁吸入系统，该系统允许小鼠自愿吸入电子烟液产生的尼古丁蒸气。结果：利用该系统，我们研究了尼古丁暴露对成年和青春期小鼠的代谢影响。成年小鼠暴露在尼古丁蒸气中10天，体重明显减轻，戒烟后迅速恢复，与人类吸烟模式相似。相比之下，青春期小鼠在尼古丁暴露期间体重没有减轻，但在戒烟后脂肪堆积增加。这些结果突出了尼古丁代谢作用的关键年龄依赖性差异，并挑战了尼古丁有助于青少年体重控制的普遍看法。结论：我们的新型电子烟液吸入模型为研究尼古丁的生理和行为效应提供了一个有价值的工具，对了解吸烟相关的健康风险和成瘾机制具有重要意义。

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来源期刊

Cell and Bioscience BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

10.70

自引率

0.00%

发文量

187

审稿时长

>12 weeks

期刊介绍： Cell and Bioscience, the official journal of the Society of Chinese Bioscientists in America, is an open access, peer-reviewed journal that encompasses all areas of life science research.