The relationship between NETosis findings and disease activity in Behçet's disease: an exploratory study.

IF 3.4 4区 医学 Q2 RHEUMATOLOGY
Erdem Bektas, Rabia Deniz, Zeliha Emrence, Sema Sirma Ekmekci, Neslihan Abaci, Shirkhan Amikishiyev, Yasemin Yalcinkaya, Bahar Artim Esen, Murat Inanc, Ahmet Gül
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Abstract

Objectives: This study aimed to investigate the relationship between the neutrophil extracellular traps (NET) and NETosis findings and disease activity in Behçet disease (BD).

Methods: The study group consisted of 30 active BD patients and 10 healthy individuals as controls. Serum and saliva samples were collected from the patients during their active and remission periods. A subset of them were followed longitudinally. Serum and saliva cf-DNA, NE, MPO, and cit-H3 levels were measured as indirect NETosis findings, subsequently the results were adjusted based on the peripheral blood neutrophil counts. Unadjusted and adjusted levels of the NETosis findings were evaluated.

Results: In active BD, unadjusted serum cf-DNA and NE levels were significantly higher than in controls, whereas adjusted serum MPO and cit-H3 levels were found to be lower. In inactive BD, unadjusted serum NE levels remained elevated compared to controls, while unadjusted serum MPO, adjusted serum MPO, and adjusted serum cit-H3 levels were lower. No significant differences were observed in salivary NETosis findings between the patients and controls. Longitudinal follow-up revealed a decrease in both unadjusted serum cf-DNA and saliva cf-DNA levels in parallel with reduced clinical activity. Saliva and unadjusted serum cf-DNA showed a positive correlation with inflammatory markers, whereas adjusted serum MPO and cit-H3 correlated negatively.

Conclusions: Indirect NETosis findings varied in relation to the systemic and/or local activity of BD patients. The changes after adjustment suggest that serum NETosis markers can be influenced by increased neutrophil turnover during the active phase of the disease.

behaperet病NETosis结果与疾病活动度的关系:一项探索性研究。
目的:本研究旨在探讨中性粒细胞胞外陷阱(NET)和NETosis在behet病(BD)中的表现和疾病活动之间的关系。方法:研究组由30例活动期BD患者和10例健康人作为对照。在患者的活跃期和缓解期采集血清和唾液样本。其中一部分人进行了纵向随访。测定血清和唾液cf-DNA、NE、MPO和cit-H3水平作为间接NETosis结果,随后根据外周血中性粒细胞计数对结果进行调整。对NETosis检查结果的未调整和调整水平进行评估。结果:活动性BD患者未调整的血清cf-DNA和NE水平显著高于对照组,而调整后的血清MPO和cit-H3水平则较对照组低。在非活动性BD中,与对照组相比,未调整的血清NE水平仍然升高,而未调整的血清MPO、调整的血清MPO和调整的血清cit-H3水平较低。唾液NETosis在患者和对照组之间没有显著差异。纵向随访显示,未调整的血清cf-DNA和唾液cf-DNA水平均随临床活动减少而下降。唾液和未调整的血清cf-DNA与炎症标志物呈正相关,而调整后的血清MPO和cit-H3呈负相关。结论:间接NETosis的结果与BD患者的全身和/或局部活动有关。调整后的变化表明,血清NETosis标志物可能受到疾病活跃期中性粒细胞周转增加的影响。
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来源期刊
CiteScore
6.10
自引率
18.90%
发文量
377
审稿时长
3-6 weeks
期刊介绍: Clinical and Experimental Rheumatology is a bi-monthly international peer-reviewed journal which has been covering all clinical, experimental and translational aspects of musculoskeletal, arthritic and connective tissue diseases since 1983.
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