CircHOMER1 in mediating porcine skeletal muscle development.

IF 3.7 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2025-09-26 DOI:10.1186/s12864-025-11991-2

Meng Li, Yuxuan Gong, Shuangji Ma, Jiayi Chen, Wanfeng Zhang, Yang Yang, Guoqing Cao, Xiaohong Guo, Bugao Li

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引用次数: 0

Abstract

Background: Skeletal muscle serves as the principal source of high-quality protein in human nutrition. Recent studies indicate that circRNAs play key roles in myogenesis by acting as competing endogenous RNAs (ceRNAs). However, the specific roles of circRNAs in myogenesis are largely unknown. Here, we attempted to systematically explore the functional role of circHOMER1 in myogenesis.

Results: The present study first demonstrated that circHOMER1 promotes the proliferation and differentiation of porcine skeletal muscle satellite cells (SMSCs). CircHOMER1 ceRNA regulatory network was constructed based on miRNA and mRNA sequencing. With immunofluorescence, dual-luciferase reporter gene, RIP, and rescue experiments, circHOMER1 was identified to promote porcine SMSCs differentiation by competitive binding miR-199b-5p to further inhibit MAP3K11 expression. Moreover, in vivo experiments revealed that circHOMER1 accelerates muscle regeneration and development.

Conclusions: Collectively, circHOMER1 promotes the differentiation of SMSCs, muscle regeneration, and muscle development. In addition, a novel circHOMER1/miR-199b-5p/MAP3K11 regulatory network in porcine myogenesis was constructed. Our results provided a basis for in-depth analysis of the role of circRNAs in myogenesis.

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CircHOMER1介导猪骨骼肌发育。

背景：骨骼肌是人类营养中优质蛋白质的主要来源。最近的研究表明，circRNAs作为竞争内源性rna （ceRNAs）在肌肉发生中发挥关键作用。然而，环状rna在肌肉发生中的具体作用在很大程度上是未知的。在这里，我们试图系统地探索circHOMER1在肌肉发生中的功能作用。结果：本研究首次证实circHOMER1可促进猪骨骼肌卫星细胞（SMSCs）的增殖和分化。基于miRNA和mRNA测序构建CircHOMER1 ceRNA调控网络。通过免疫荧光、双荧光素酶报告基因、RIP和救援实验，我们发现circHOMER1通过竞争性结合miR-199b-5p促进猪SMSCs分化，进一步抑制MAP3K11的表达。此外，体内实验显示circHOMER1促进肌肉再生和发育。综上所述，circHOMER1促进了SMSCs的分化、肌肉再生和肌肉发育。此外，我们还构建了一个新的circHOMER1/miR-199b-5p/MAP3K11在猪肌肉发生中的调控网络。我们的结果为深入分析环状rna在肌肉发生中的作用提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.