Multi-omics analysis reveals the synergistic and intergenerational toxicity of Cr(VI) and chlorfenapyr on zebrafish: disruption of gut-liver axis and mitochondrial impairment

IF 6 3区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Sciences Europe Pub Date : 2025-09-26 DOI:10.1186/s12302-025-01198-3

Dou Wang, Ran Yi, Ruike Wang, Kan Shao, Chen Chen, Liangang Mao, Xinju Liu, Ting Luo, Xinquan Wang, Yanhua Wang

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引用次数: 0

Abstract

Background

Concurrent hexavalent chromium [Cr(VI)] and chlorfenapyr contamination in aquatic environments poses risks to environmental organisms. A parental zebrafish (Danio rerio) exposure model with multi-omics analyses was used to elucidate molecular and transgenerational effects.

Results

Combined exposure decreased intestinal tight junction transcription (zo-1, and occludin), caused morphological damage, and activated pro-inflammatory cytokines (il-1β, il-6, il-8, and TNF-α). 16S rRNA sequencing revealed reduced butyrate-producing bacteria (e.g., Coprococcus), while untargeted metabolomics showed declines in phospholipid precursors (choline, and glycerophosphocholine), implicating disrupted gut barrier metabolism. Hepatic transcriptomics identified downregulation of oxidative phosphorylation components (atp5l, atp5mc3b, atp5po, and ndufs7) and antioxidant enzymes (gpx, and mn-sod), correlating with decreased ATP, mitochondrial membrane potential, and increased apoptosis. F₁ offspring exhibited aberrations in gene transcriptions associated with oxidative phosphorylation and inflammation, suggesting the presence of intergenerational toxicity.

Conclusions

These results highlighted the gut-liver axis and mitochondrial function as targets for molecular biomarkers and inform eco-safety regulation addressing chemical mixture hazards.

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多组学分析揭示了铬（VI）和氯虫腈对斑马鱼的协同毒性和代际毒性：肠-肝轴破坏和线粒体损伤

水生环境中六价铬[Cr(VI)]和杀虫腈的同时污染会对环境生物造成危害。本研究以亲代斑马鱼（Danio rerio）暴露模型为研究对象，采用多组学分析来阐明分子效应和跨代效应。结果联合暴露降低肠道紧密连接转录（zo-1和occludin），造成形态学损伤，激活促炎细胞因子（il-1β、il-6、il-8和TNF-α）。16S rRNA测序显示产生丁酸的细菌（如粪球菌）减少，而非靶向代谢组学显示磷脂前体（胆碱和甘油磷胆碱）下降，暗示肠道屏障代谢被破坏。肝脏转录组学发现氧化磷酸化成分（atp5l、atp5mc3b、atp5po和ndufs7）和抗氧化酶（gpx和mn-sod）下调，与ATP降低、线粒体膜电位降低和细胞凋亡增加相关。F₁后代表现出与氧化磷酸化和炎症相关的基因转录异常，表明存在代际毒性。结论这些结果突出了肠肝轴和线粒体功能作为分子生物标志物的目标，并为解决化学混合物危害的生态安全调控提供了信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Sciences Europe Environmental Science-Pollution

CiteScore

11.20

自引率

1.70%

发文量

110

审稿时长

13 weeks

期刊介绍： ESEU is an international journal, focusing primarily on Europe, with a broad scope covering all aspects of environmental sciences, including the main topic regulation. ESEU will discuss the entanglement between environmental sciences and regulation because, in recent years, there have been misunderstandings and even disagreement between stakeholders in these two areas. ESEU will help to improve the comprehension of issues between environmental sciences and regulation. ESEU will be an outlet from the German-speaking (DACH) countries to Europe and an inlet from Europe to the DACH countries regarding environmental sciences and regulation. Moreover, ESEU will facilitate the exchange of ideas and interaction between Europe and the DACH countries regarding environmental regulatory issues. Although Europe is at the center of ESEU, the journal will not exclude the rest of the world, because regulatory issues pertaining to environmental sciences can be fully seen only from a global perspective.