Design and Numerical Analysis of a Single Inlet-Based Spiral Microfluidic Chip for Separation of Circulating Tumor Cells and Blood Plasma Using the Inertial Focusing Approach

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-05-22 DOI:10.1007/s10337-025-04411-w

Writtick Pakhira, R. Kumar, Khalid Mohd. Ibrahimi, Rituraj Bhattacharjee

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引用次数: 0

Abstract

Circulating tumor cells (CTCs) and blood plasma both work as biomarkers for the initial detection and diagnosis of metastatic cancers. Nowadays, with the development of microscale devices, for the separation of CTCs and blood plasma from normal cells, one of the most effective techniques is inertial focusing using microfluidic chips. The present research work reported a single inlet and single loop-based microfluidic chip for simultaneous separation of PC3 (prostate cancer) CTCs and blood plasma from normal blood cells using the inertial focusing approach. Two different sized PC3 CTCs and different inlet flow velocities corresponding to different Reynolds numbers are explored to accomplish optimum separation performance of the chip. Three-dimensional numerical simulations were executed with COMSOL Multiphysics 5.4 software and finite element method analysis. It has been confirmed that 100 % separation purity and cell recovery were achieved for both 17 µm and 20 µm PC3 CTCs at the Reynolds number of 67 and 65.67, respectively. The microfluidic chip also achieved a maximum plasma recovery of 54.87 % with 100 % purity.

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惯性聚焦法分离循环肿瘤细胞与血浆的单进气道螺旋微流控芯片设计与数值分析

循环肿瘤细胞（CTCs）和血浆都可以作为转移性癌症初始检测和诊断的生物标志物。目前，随着微尺度设备的发展，从正常细胞中分离ctc和血浆，最有效的技术之一是利用微流控芯片进行惯性聚焦。本研究报道了一种基于单入口、单回路的微流控芯片，用于同时分离前列腺癌ctc和正常血细胞中的血浆。探索了两种不同尺寸的PC3 ctc和不同雷诺数对应的不同进口流速，以实现芯片的最佳分离性能。采用COMSOL Multiphysics 5.4软件进行三维数值模拟，并进行有限元分析。在雷诺数为67和65.67时，17µm和20µm PC3 ctc的分离纯度和细胞回收率均达到100%。微流控芯片在纯度为100%的情况下，等离子体回收率最高可达54.87%。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.