Determination of Major Anions in Human Serum by Capillary Zone Electrophoresis

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-06-23 DOI:10.1007/s10337-025-04415-6

Evgeniya V. Polyakova, Kirill N. Narozhnykh, Sofiya E. Romanova, Tatyana V. Skiba

引用次数: 0

Abstract

This study presents an optimized method for determining major anions (chloride, sulfate, phosphate, citrate, and lactate) in human serum using capillary zone electrophoresis (CZE) with indirect photometric detection. A comparative analysis was conducted to evaluate the effectiveness of an electroosmotic flow (EOF) modifier (cetyltrimethylammonium hydroxide—CTAOH) and an ion-pairing reagent (tetrabutylammonium hydroxide—TBAOH). The optimal electrolyte composition (10 mM chromate, 16 mM DEA, 0.5 mM CTAOH) was determined, ensuring high separation quality and detection sensitivity. An improved sample preparation method, involving protein precipitation with acetonitrile, was implemented. Achieved limits of detection were 6 mg/L for sulfate, 12 mg/L for phosphate, 10 mg/L for citrate, and 16 mg/L for lactate. The method’s validation characteristics (spike-recovery of 90–111%, relative standard deviation (RSD) ≤ 10%) demonstrate high accuracy and reproducibility.

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毛细管区带电泳法测定人血清中主要阴离子

本研究提出了一种毛细管区带电泳间接光度法测定人血清中主要阴离子（氯化物、硫酸盐、磷酸盐、柠檬酸盐和乳酸盐）的优化方法。对比分析了电渗透流（EOF）改性剂（十六烷基三甲基氢化铵- ctaoh）和离子配对试剂（四丁基氢化铵- tbaoh）的有效性。确定了最佳电解液组成（10 mM铬酸盐、16 mM DEA、0.5 mM CTAOH），保证了较高的分离质量和检测灵敏度。改进了用乙腈沉淀蛋白质的样品制备方法。实现的检出限分别为：硫酸盐6 mg/L、磷酸盐12 mg/L、柠檬酸盐10 mg/L、乳酸16 mg/L。该方法的验证特性（峰回收率为90 ~ 111%，相对标准偏差(RSD)≤10%）具有较高的准确性和重复性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.