Analyzing the Volatile Flavor Components in Shanyao Yogurts

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2025-04-09 DOI:10.1007/s10337-025-04408-5

Yu Zhang, Fuli Li, Jiahui Liu, Jingchun Wang, Chaozheng Zhang

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Abstract

To comprehensively investigate the flavor characteristics of Shanyao yogurt, headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC–MS) and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) techniques were employed to analyze volatile flavor compounds(VFCs) in Shanyao yogurts fermented with Lactobacillus rhamnosus and Lactobacillus plantarum. The findings of the two techniques revealed variations in both components and concentrations of VFCs in the same sample. HS-SPME-GC-MS exhibited excellent performance in detecting acidic substances, while HS-GC-IMS demonstrated advantages in analyzing ester substances. Only 4 compounds were identified by both methods in Shanyao yogurt fermented by L. plantarum; whereas 7 compounds were identified by both methods in Shanyao yogurt fermented by L. rhamnosus. This research underscored the benefits of employing diverse analytical techniques for studying yogurt samples, as integrating multiple approaches provided a more comprehensive understanding of VFCs in Shanyao yogurt, thereby offering robust support for investigating VFCs in similar products.

Graphical Abstract

Abstract Image

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山药酸奶挥发性风味成分分析

为全面研究山药酸奶的风味特征，采用顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）和顶空气相色谱-离子迁移谱联用（HS-GC-IMS）技术对鼠李糖乳杆菌和植物乳杆菌发酵的山药酸奶中的挥发性风味物质进行了分析。两种技术的发现揭示了同一样品中vfc的成分和浓度的变化。HS-SPME-GC-MS在检测酸性物质方面表现优异，而HS-GC-IMS在分析酯类物质方面表现优异。植物乳杆菌发酵的山药酸奶中，两种方法均鉴定出4种化合物；两种方法均鉴定出山药酸奶中的7个化合物。本研究强调了采用多种分析技术研究酸奶样品的好处，因为综合多种方法可以更全面地了解山药酸奶中的vfc，从而为研究类似产品中的vfc提供有力的支持。图形抽象

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.