“Therapeutic Potential of Boswellic Acid-Loaded Nanostructured Lipid Carriers in Aloe Gel: A Novel Approach for Effective Gout Management on Wistar Rats”
Sahil Naik, Kishori P. Sutar, Sankalp S. Sammasagi, Prakash Biradar, Anvitha Sequeira, Arun Jadhav
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引用次数: 0
Abstract
Purpose
The present study aims to develop, optimize, and evaluate 3-acetyl-11-keto-ꞵ-Boswellic acid-loaded nanostructured lipid carriers incorporated into an aloe-based gel for effective gout management. The objective was to enhance drug permeation, achieve sustained release, and overcome challenges associated with oral delivery of 3-acetyl-11-keto-ꞵ-Boswellic acid.
Methods
Nanostructured lipid carriers were prepared using the micro-emulsion method, employing a Box-Behnken full factorial design with varying concentrations of stearic acid, oleic acid, and Tween 80. Optimization was based on particle size, zeta potential, entrapment efficiency, Polydispersibility Index, drug content, and in vitro release. Transmission electron microscopy confirmed the nano-sized, well-dispersed particles. The optimized batch (F5) was incorporated into a 1.5% w/v aloe-based gel, which was subsequently assessed for pH, viscosity, spreadability, in vitro diffusion, in vivo dermal irritancy, and efficacy against Monosodium Urate-induced gout.
Results
The optimized Nanostructured lipid carriers exhibited a particle size of 110.1 nm, zeta potential of -32.1 mV, entrapment efficiency of 80.3%, and drug content of 78.21 ± 1.57%. The gel formulation showed a viscosity of 781 ± 6.16 cps, spreadability of 7.67 ± 0.0942 g.cm/sec, and pH of 6.24 ± 0.05. In vitro drug release was 85.96% over 12 h, with the gel demonstrating 83.01% diffusion. In vivo studies confirmed significant therapeutic efficacy, non-irritancy, and enhanced mobility in treated subjects.
Conclusion
The boswellic acid-loaded Nanostructured lipid carriers aloe gel offers a promising strategy for gout management, providing enhanced permeation, sustained release, and minimal dermal irritation. Stability studies further support its potential for therapeutic application.
期刊介绍:
The Journal of Pharmaceutical Innovation (JPI), is an international, multidisciplinary peer-reviewed scientific journal dedicated to publishing high quality papers emphasizing innovative research and applied technologies within the pharmaceutical and biotechnology industries. JPI''s goal is to be the premier communication vehicle for the critical body of knowledge that is needed for scientific evolution and technical innovation, from R&D to market. Topics will fall under the following categories:
Materials science,
Product design,
Process design, optimization, automation and control,
Facilities; Information management,
Regulatory policy and strategy,
Supply chain developments ,
Education and professional development,
Journal of Pharmaceutical Innovation publishes four issues a year.