Utilizing a microfluidic approach for high-throughput synthesis of drug encapsulated Nanoliposomes

IF 5.2 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-09-20 DOI:10.1016/j.molliq.2025.128570

Vidhi Jain , Vishwa Gandhi , Ram Das , Beena Singh , Shashi Prakash , Sanjay Singh , Dharmesh Varade

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Abstract

Microfluidic platform was utilized for producing nanoliposomes using egg phosphatidylcholine (E-PC) and cholesterol (CH) by efficiently leveraging the mixing properties of microfluidic channels in a range of designs, including ‘Serpentine’, ‘Solid serpentine’, and ‘Koch fractal’. These microfluidic channels were made with computer-aided design (CAD) software and then transferred onto acrylic sheets using a CO₂ laser. Along with carefully assessing the relative sizes and topologies of the channels, liquid flow parameters, and initial lipid concentrations, the characteristics of nanoliposomes were investigated using small-angle neutron scattering (SANS), dynamic light scattering (DLS), and high resolution transmission electron microscopy (HR-TEM). We were able to control the nanoliposomes dimensions within the preferred range (hydrodynamic diameter: 70–200 nm, PDI: less than 0.3). The operational framework of the proposed methodologies and the acquired results were readily adapted to the continuous, fast, and regulated creation of the nanoliposomes. Additionally, anticancer medications such as doxorubicin and genistein were successfully encapsulated in nanoliposomes, which demonstrated efficacious activity.

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利用微流控技术高通量合成药物包封纳米脂质体

微流控平台利用鸡蛋磷脂酰胆碱（E-PC）和胆固醇（CH）生产纳米脂质体，有效利用了一系列设计的微流控通道的混合特性，包括“蛇形”、“固体蛇形”和“Koch分形”。这些微流体通道是用计算机辅助设计（CAD）软件制作的，然后用二氧化碳激光转移到丙烯酸片上。除了仔细评估通道的相对大小和拓扑结构、液体流动参数和初始脂质浓度外，还使用小角中子散射（SANS）、动态光散射（DLS）和高分辨率透射电子显微镜（HR-TEM）研究了纳米脂质体的特性。我们能够将纳米脂质体的尺寸控制在理想的范围内（水动力直径：70-200 nm， PDI：小于0.3）。所提出的方法的操作框架和所获得的结果很容易适应于纳米脂质体的连续、快速和有调节的产生。此外，抗癌药物如阿霉素和染料木素被成功地封装在纳米脂质体中，并显示出有效的活性。

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.