SLIMPHONY: A SLIM-Based Instrument That Orchestrates Complex Ion Mobility-Mass Spectrometry Experiments.

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
AnneClaire Wageman, Addison E Roush, Yuan Feng, Matthew F Bush
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引用次数: 0

Abstract

The inherent heterogeneity of biological macromolecules offers a unique challenge for analysis. The combination of ion mobility (IM) and mass spectrometry (MS) is sensitive to the size, shape, and dynamics of, for example, proteins and their complexes. Combining multiple dimensions of ion mobility and mass spectrometry (IM-IM-MS) while leveraging unique gas-phase manipulations between dimensions has great potential for increasing the information content for challenging analytes. Here, we introduce an instrument, SLIMPHONY, which was built using the Structures for Lossless Ion Manipulations (SLIM) architecture. SLIMPHONY is unique in that eight independently controlled traveling-wave regions work in concert to enable complex, multidimensional separations. Single-dimension IM-MS experiments were used to separate a mixture of protein and protein-complex ions and demonstrate that the peak-to-peak resolution increases roughly with the square root of the separation length for a pair of hexakis(fluoroalkoxy)phosphazine ions. Ion selection and trapping between dimensions was then used to probe the gas-phase unfolding of a subpopulation of ubiquitin ions. Finally, by varying the guard potential used to confine ions, we demonstrate tunable activation of ubiquitin subpopulations, which we analyzed using IM separations of various lengths. With the ability to select and activate ions in multiple regions, to vary the number of dimensions of IM, and to control the length of IM separation, SLIMPHONY is a flexible platform for characterizing protein ions.

SLIMPHONY:一种基于细长的仪器,可协调复杂离子迁移-质谱实验。
生物大分子固有的异质性为分析提供了一个独特的挑战。离子迁移率(IM)和质谱(MS)的结合对诸如蛋白质及其复合物的大小、形状和动力学非常敏感。结合离子迁移率和质谱(IM-IM-MS)的多个维度,同时利用不同维度之间独特的气相操作,对于增加具有挑战性的分析物的信息内容具有巨大的潜力。在这里,我们介绍了一种仪器,SLIMPHONY,它是使用结构无损离子操作(SLIM)架构构建的。SLIMPHONY的独特之处在于,八个独立控制的行波区域协同工作,实现了复杂的多维分离。单维IM-MS实验用于分离蛋白质和蛋白质复合物离子的混合物,结果表明,对于一对六烷基(氟烷氧基)磷hazine离子,峰间分辨率大致随分离长度的平方根而增加。离子选择和捕获之间的维度,然后被用来探测气相展开的亚群泛素。最后,通过改变用于限制离子的保护电位,我们证明了泛素亚群的可调激活,我们使用不同长度的IM分离来分析。sliphony具有在多个区域选择和激活离子的能力,可以改变IM的维数,并控制IM分离的长度,是表征蛋白质离子的灵活平台。
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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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