Molecular Species of Membrane Lipids of the Sea Anemone Exaiptasia diaphana and Its Symbionts

IF 1.7 4区化学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Russian Journal of Bioorganic Chemistry Pub Date : 2025-07-28 DOI:10.1134/S106816202504034X

E. T. Bizikashvili, S. A. Kozlovskiy, E. V. Ermolenko, K. V. Efimova, T. V. Sikorskaya

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Abstract

Objective: The molecular species of membrane lipids of the sea anemone Exaiptasia diaphana and the molecular species of glycolipids of its symbionts were studied. Methods: Lipidomic analysis was performed using high-performance liquid chromatography with mass spectrometric detection. Results and Discussion: A total of 82 molecular species of E. diaphana glycerophospholipids were identified, the main ones being 16:0/22:6 cholineglycerophospholipid (PC), 18:1e/20:4 and 18:1e/20:5 ethanolaminglycerophospholipids (PE), 18:0/22:4 serineglycerophospholipid (PS), 18:0/22:4 inositolglycerophospholipid (PI), and 18:2b/16:0 ceramidaminoethylphosphonate (CAEP). Thirty-six molecular species of glycolipids were identified in symbionts. The main molecular species were 18:4/18:5 monogalactosyldiacylglycerol (MGDG), 18:3/18:5 and 18:4/18:4 digalactosyldiacylglycerols (DGDG), and 14:0/16:0 sulfoquinovosyldiacylglycerol (SQDG). Molecular genetic analysis revealed that all E. diaphana colonies contained the following dinoflagellates: Breviolum minutum, Cladocopium thermophilum, and Gerakladium endoclionum. Conclusions: The profile of lipid molecular species of the coral host can act as a chemotaxonomic feature, and galactolipids of symbionts indicate resistance to changes in seawater temperature. This study contributes to the development of lipidomics of marine organisms of the phylum Cnidaria.

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海葵膜脂的分子种类及其共生体

目的：研究海葵（Exaiptasia diaphana）膜脂的分子种类及其共生体糖脂的分子种类。方法：采用高效液相色谱-质谱法进行脂质组学分析。结果与讨论：共鉴定出82个分子种，主要有16:0/22:6胆碱甘油磷脂（PC）、18:1e/20:4和18:1e/20:5乙醇酰胺甘油磷脂（PE）、18:0/22:4丝氨酸甘油磷脂（PS）、18:0/22:4肌醇甘油磷脂（PI）和18:2b/16:0神经酰胺酰膦酸酯（CAEP）。共鉴定出36个糖脂分子种。主要分子种类为18:4/18:5单半乳糖二酰基甘油（MGDG）、18:3/18:5和18:4/18:4双半乳糖二酰基甘油（DGDG）和14:0/16:0磺基喹啉二酰基甘油（SQDG）。分子遗传分析表明，所有菌落均含有短鞭毛菌（Breviolum minutum）、嗜热枝鞭毛菌（Cladocopium thermoophilum）和内生叶鞭毛菌（Gerakladium endodoclionum）。结论：珊瑚寄主的脂质分子种类可以作为一种化学分类特征，共生体的半乳脂质表明其对海水温度变化的抗性。该研究有助于刺胞门海洋生物脂质组学的发展。

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来源期刊

Russian Journal of Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

1.80

自引率

10.00%

发文量

118

审稿时长

3 months

期刊介绍： Russian Journal of Bioorganic Chemistry publishes reviews and original experimental and theoretical studies on the structure, function, structure–activity relationships, and synthesis of biopolymers, such as proteins, nucleic acids, polysaccharides, mixed biopolymers, and their complexes, and low-molecular-weight biologically active compounds (peptides, sugars, lipids, antibiotics, etc.). The journal also covers selected aspects of neuro- and immunochemistry, biotechnology, and ecology.