Identification of novel H2A histone variants across diverse clades of algae

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ellyn Rousselot, Zofia Nehr, Jean-Marc Aury, France Denoeud, J. Mark Cock, Leïla Tirichine, Céline Duc
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引用次数: 0

Abstract

Histones are among the most conserved proteins in eukaryotes. They not only ensure DNA compaction in the nucleus but also participate in epigenetic regulation of gene expression. These key epigenetic players are divided into replication-coupled histones, expressed during the S-phase, and replication-independent variants, expressed throughout the cell cycle. Compared with other core histones, H2A proteins exhibit a high level of variability but the characterization of algal H2A variants remains very limited. In this study, we exploit genome and transcriptome data from 22 species to identify H2A variants in brown seaweeds. Combined analyses of phylogenetic data, synteny and protein motifs enable us to reveal the presence of new H2A variants as well as their evolutionary history. We identify three new H2A variants: H2A.N, H2A.O and H2A.E. In brown seaweeds, the H2A.E and H2A.O variants arose from the same monophyletic clade while the H2A.N variant emerged independently. Moreover, the H2A.E variant seems to have a shared ancestry with RC H2A while the H2A.O variant has an H2A.X-characteristic signature without being orthologous to this variant. Based on mass spectrometry, we identify distinct epigenetic marks on these H2A variants. Finally, the H2A.Z, H2A.N and H2A.O from brown seaweeds are ubiquitously expressed while expression of H2A.E has tissue-specific patterns, especially in reproductive tissues. We thus hypothesize that H2A.O and H2A.X might have convergent functions while H2A.E might fulfil some functions of replication-coupled H2As and/or compensate for the absence of repressive histone marks along with H2A.N.
在不同的藻类分支中鉴定新的H2A组蛋白变异
组蛋白是真核生物中最保守的蛋白质之一。它们不仅保证DNA在细胞核内的压实,还参与基因表达的表观遗传调控。这些关键的表观遗传参与者分为复制偶联组蛋白(在s期表达)和复制非依赖性组蛋白(在整个细胞周期中表达)。与其他核心组蛋白相比,H2A蛋白表现出高水平的可变性,但藻类H2A变异的表征仍然非常有限。在这项研究中,我们利用22种褐海藻的基因组和转录组数据来鉴定H2A变异。系统发育数据、合成和蛋白质基序的综合分析使我们能够揭示新的H2A变异的存在及其进化史。我们确定了三种新的H2A变体:N, H2A。O和h2a。在棕色海藻中,H2A。E和H2A。O型变异来自同一单系进化枝,而H2A型变异来自同一单系进化枝。N个变体独立出现。此外,H2A。E变体似乎与RC H2A有共同的祖先,而H2A。O型变体有H2A。x特征签名而不与此变体同源。基于质谱分析,我们在这些H2A变异上发现了不同的表观遗传标记。最后是H2A。Z, H2A。N和H2A。O在褐藻中普遍表达,而H2A在褐藻中表达。E具有组织特异性模式,特别是在生殖组织中。因此我们假设H2A。O和H2A。X可能有收敛函数,而H2A。E可能完成复制偶联H2As的某些功能和/或补偿与H2A.N一起缺失的抑制性组蛋白标记。
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来源期刊
Genome Biology
Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
21.00
自引率
3.30%
发文量
241
审稿时长
2 months
期刊介绍: Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.
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