Fueling chromosomal gene diversification and artificial evolution with CRISPR

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genome Biology Pub Date : 2025-09-23 DOI:10.1186/s13059-025-03756-7

Ruiying Zhu, Chuanhong Ren, Zehua Bao

引用次数: 0

Abstract

Gene diversification is an effective approach to massively dissecting variant functions and evolving sequences when paired with an appropriate assay. In vitro mutagenesis and ectopic gene expression, however, fail to simulate the endogenous regulatory environment of the variants. The development of clustered, regularly interspaced short palindromic repeats (CRISPR) systems has greatly boosted the efficiency of targeted gene diversification in various species. Here, we review recent CRISPR-assisted methods for chromosomal gene diversification and artificial evolution, focusing on the advantages and limitations of each approach, and propose possible strategies to overcome current limitations and directions in future technology development.

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利用CRISPR促进染色体基因多样化和人工进化

基因多样化是大规模解剖变异功能和进化序列的有效方法，当与适当的分析配对时。然而，体外诱变和异位基因表达无法模拟变异的内源性调控环境。聚集的、有规律间隔的短回文重复序列（CRISPR）系统的发展极大地提高了不同物种中靶向基因多样化的效率。本文综述了近年来crispr辅助染色体基因多样化和人工进化的方法，重点分析了每种方法的优点和局限性，并提出了克服当前局限性的可能策略和未来技术发展的方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

21.00

自引率

3.30%

发文量

241

审稿时长

2 months

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