Dynamics of single enzymes confined inside a nanopore.

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nicole Stéphanie Galenkamp, Marco van den Noort, Giovanni Maglia
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引用次数: 0

Abstract

Enzymes are powerful catalysts that perform chemical reactions with remarkable speed and specificity. Their intrinsic dynamics often play a crucial role in determining their catalytic properties. To achieve a comprehensive understanding of enzymes, a diverse and sophisticated experimental toolbox capable of studying enzyme dynamics at the single-molecule level is necessary. In this review, we discuss nanopore technology as an emerging and powerful platform in single-molecule enzymology. We demonstrate how nanopores can be employed to probe enzyme dynamics in real-time, and we highlight how these studies have contributed to fundamentally and quantitatively elucidating enzymological concepts, such as allostery and hysteresis. Finally, we explore the potentials and limitations of nanopores in advancing single-molecule enzymology. By presenting the unique possibilities offered by nanopores, we aim to inspire the integration of this technology into future enzymology research.

纳米孔内单个酶的动力学。
酶是强大的催化剂,能以惊人的速度和特异性进行化学反应。它们的内在动力学往往在决定其催化性能方面起着至关重要的作用。为了实现对酶的全面了解,需要一个能够在单分子水平上研究酶动力学的多样化和复杂的实验工具箱。在这篇综述中,我们讨论了纳米孔技术作为一个新兴的和强大的平台在单分子酶学。我们展示了如何利用纳米孔实时探测酶动力学,并强调了这些研究如何从根本上和定量地阐明酶学概念,如变构和滞后。最后,我们探讨了纳米孔在推进单分子酶学方面的潜力和局限性。通过展示纳米孔提供的独特可能性,我们的目标是激发这项技术整合到未来的酶学研究中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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