Proteomic Analysis of Sputum from Patients with Active Tuberculosis.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Proteomes Pub Date : 2025-09-12 DOI:10.3390/proteomes13030043

Endrei Marcantonio, Amy M Woron, A Christian Whelen, Sladjana Prisic

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引用次数: 0

Abstract

Background: Patients with pulmonary tuberculosis (TB) typically produce sputa, which are used to identify the pathogen. Sputum also contains host proteins that may aid in diagnosis. We hypothesized that sputa from TB patients will have unique proteomes when compared to other lung diseases.

Methods: Sputa were collected from 219 patients with suspected TB. Neutrophil-derived protein calprotectin (CP), which was used as a marker for lung damage, was quantified and compared between TB and non-TB groups. Three sputa with high or low CP from each group were selected and analyzed using label-free proteomics.

Results: There was no difference in CP amounts between TB and non-TB groups. However, TB samples had other differentially abundant neutrophil-associated proteins. Compared to low CP, samples with high CP had much smaller number of proteins that could differentiate between TB and non-TB groups. Only two proteins, MUC5AC and MMP8, were more abundant in TB samples, regardless of CP levels.

Conclusions: Our findings suggest that TB sputa may have unique proteomes that depend on CP levels, which should be further validated due to the small sample size. Therefore, controlled and more advanced TB may need a different set of biomarkers to reliably distinguish TB from other lung diseases.

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活动性肺结核患者痰液的蛋白质组学分析。

背景：肺结核（TB）患者通常会产生痰液，痰液可用于鉴定病原体。痰液也含有宿主蛋白，可能有助于诊断。我们假设与其他肺部疾病相比，结核病患者的痰液将具有独特的蛋白质组。方法：对219例疑似结核患者进行痰检。作为肺损伤标志物的中性粒细胞衍生蛋白钙保护蛋白（CP）被量化，并在结核病组和非结核病组之间进行比较。每组各选择3只高或低CP的肉芽，采用无标记蛋白质组学方法进行分析。结果：结核组与非结核组CP含量无显著性差异。然而，结核样品有其他差异丰富的中性粒细胞相关蛋白。与低CP相比，具有高CP的样品具有可区分结核病和非结核病组的蛋白质数量要少得多。无论CP水平如何，只有MUC5AC和MMP8两种蛋白在结核样品中含量更高。结论：我们的研究结果表明结核痰液可能具有依赖于CP水平的独特蛋白质组，由于样本量小，这一点有待进一步验证。因此，控制和更严重的结核病可能需要一套不同的生物标志物来可靠地将结核病与其他肺部疾病区分开来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Proteomes Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

11 weeks

期刊介绍： Proteomes (ISSN 2227-7382) is an open access, peer reviewed journal on all aspects of proteome science. Proteomes covers the multi-disciplinary topics of structural and functional biology, protein chemistry, cell biology, methodology used for protein analysis, including mass spectrometry, protein arrays, bioinformatics, HTS assays, etc. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers. Scope: -whole proteome analysis of any organism -disease/pharmaceutical studies -comparative proteomics -protein-ligand/protein interactions -structure/functional proteomics -gene expression -methodology -bioinformatics -applications of proteomics