Preparation and characterization of DeSC modified using succinic anhydride

Abstract

Based on the weakly acidic pH of the microenvironment of diseased tissues and the fact that glutathione content is several times higher than that of normal tissues, a pH/oxide-reduced drug slow-release carrier was designed using dextran (Dex) as a matrix.

The carrier material DeSC was prepared by carboxylating Dex using SA with dextran as a substrate, followed by amidation reaction with Cys, and self-assembled into nanoparticles using solution volatilization method. Using Cur as the drug model, the maximum values of 14.06 ± 0.37 % and 54.54 ± 1.7 % w were achieved when the drug loading and encapsulation rates were carried out using the stirring method, and the maximum values of 13.75 ± 0.48 % and 68.18 ± 3.33 % were achieved when the drug delivery ratio was 1:5. In the in vitro simulated drug release experiments, the cumulative drug release rate of Cur@DeSC increased with decreasing pH, reaching a maximum of 48.2 ± 2.04 % at pH = 5.5; The haemolysis rate was lower than 5 % and the cell survival rate was higher than 85 %, indicating that DeSC nanoparticles have a good biosafety profile.