Synergistic and divergent effects of dietary sugars on hepatocyte viability and steatosis

IF 2.7 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2026-01-01 Epub Date: 2025-09-18 DOI:10.1016/j.tiv.2025.106148

Ajay Kumar , Garhima Arora , Deepika Kumari , Harish Changotra , Samrat Chatterjee

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引用次数: 0

Abstract

Our study explores the effects of glucose, fructose, and sucrose on hepatocyte health, focusing on cell viability and lipid accumulation. Hepatocyte cells (HepG2) were exposed to a range of concentrations of glucose, fructose and sucrose, both individually and in combination, over 24, 48, and 72 h. Cell viability was monitored using the MTT assay, while lipid accumulation was estimated fluorometrically using Nile red staining. Our findings suggest that higher concentrations of fructose significantly improves cell viability in a dose-dependent manner, especially in hepatocyte cells exposed to fructose for extended duration. Additionally, a time-dependent increase in lipid accumulation was seen, particularly in cells exposed to fructose. Interestingly, enhanced hepatocyte viability was noted with combinations of glucose with fructose or sucrose, without detrimental effects. Lipid accumulation was more pronounced with glucose-fructose combinations. These results provide insights into the differential effects of dietary sugars on liver health and metabolism, with implications for understanding metabolic disorders like MASLD and MASH.

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膳食糖对肝细胞活力和脂肪变性的协同和发散效应。

我们的研究探讨了葡萄糖、果糖和蔗糖对肝细胞健康的影响，重点是细胞活力和脂质积累。将肝细胞（HepG2）单独或联合暴露于不同浓度的葡萄糖、果糖和蔗糖中，时间分别为24、48和72 h。使用MTT法监测细胞活力，同时使用尼罗红染色法估计脂质积累。我们的研究结果表明，高浓度的果糖以剂量依赖的方式显著提高细胞活力，特别是在长时间暴露于果糖的肝细胞中。此外，脂质积累的时间依赖性增加，特别是在暴露于果糖的细胞中。有趣的是，葡萄糖与果糖或蔗糖的组合可以增强肝细胞活力，但没有有害影响。脂质积累在葡萄糖-果糖组合中更为明显。这些结果为了解膳食糖对肝脏健康和代谢的不同影响提供了见解，对理解MASLD和MASH等代谢紊乱具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.