Leveraging DNA repair mechanisms in genetically engineered biocontrol

IF 4.8 1区农林科学 Q1 BIOLOGY

Current opinion in insect science Pub Date : 2025-09-18 DOI:10.1016/j.cois.2025.101437

Joseph S Romanowski , Christian E Ogaugwu , Zach N Adelman

引用次数: 0

Abstract

Genetic biocontrol strategies such as gene drives are garnering much attention due to the great potential to achieve population control at unprecedented speed and precision. Several endogenous DNA repair pathways can facilitate or counteract the success of gene drives. Current studies focus on HDR and NHEJ pathways and employ various detection and analytical methods to assess editing events due to these DNA repair pathways. However, other DNA repair pathways, such as MMEJ and SSA, could equally be leveraged to design better gene drives. More methods for predicting, detecting, and analyzing editing events due to these diverse pathways are needed. This review explores the currently available tools and packages to assist in this venture.

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利用基因工程生物防治中的DNA修复机制。

基因驱动等遗传生物防治策略由于具有以前所未有的速度和精度实现人口控制的巨大潜力而受到广泛关注。几种内源性DNA修复途径可以促进或抵消基因驱动的成功。目前的研究主要集中在HDR和NHEJ通路上，并采用各种检测和分析方法来评估这些DNA修复通路引起的编辑事件。然而，其他DNA修复途径，如MMEJ和SSA，同样可以用来设计更好的基因驱动。由于这些不同的途径，需要更多的方法来预测、检测和分析编辑事件。这篇综述探讨了目前可用的工具和包，以协助进行这项工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in insect science BIOLOGYECOLOGYENTOMOLOGY-ECOLOGY

CiteScore

10.40

自引率

1.90%

发文量

113

期刊介绍： Current Opinion in Insect Science is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up–to–date with the expanding volume of information published in the field of Insect Science. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following 11 areas are covered by Current Opinion in Insect Science. -Ecology -Insect genomics -Global Change Biology -Molecular Physiology (Including Immunity) -Pests and Resistance -Parasites, Parasitoids and Biological Control -Behavioural Ecology -Development and Regulation -Social Insects -Neuroscience -Vectors and Medical and Veterinary Entomology There is also a section that changes every year to reflect hot topics in the field. Section Editors, who are major authorities in their area, are appointed by the Editors of the journal. They divide their section into a number of topics, ensuring that the field is comprehensively covered and that all issues of current importance are emphasized. Section Editors commission articles from leading scientists on each topic that they have selected and the commissioned authors write short review articles in which they present recent developments in their subject, emphasizing the aspects that, in their opinion, are most important. In addition, they provide short annotations to the papers that they consider to be most interesting from all those published in their topic over the previous year.