Comparative evaluation of polyhydroxyalkanoates recovery methods for a mixed microbial culture derived from waste activated sludge

IF 3 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Journal of Material Cycles and Waste Management Pub Date : 2025-05-12 DOI:10.1007/s10163-025-02242-3

Daisuke Inoue, Kohei Miwa, Michihiko Ike

{"title":"Comparative evaluation of polyhydroxyalkanoates recovery methods for a mixed microbial culture derived from waste activated sludge","authors":"Daisuke Inoue, Kohei Miwa, Michihiko Ike","doi":"10.1007/s10163-025-02242-3","DOIUrl":null,"url":null,"abstract":"<div><p>The production of polyhydroxyalkanoates (PHAs) using a mixed microbial culture (MMC) derived from waste activated sludge (WAS) is a promising strategy for converting wastewater treatment plants into biorefinery facilities. Despite many studies on PHA production, the recovery of PHA stored in WAS-derived MMC has received less attention. In this study, we comparatively evaluated three solvent extraction, three cellular digestion, and two mechanical disruption methods to determine their effectiveness in the recovery of PHA from WAS-derived MMC. Chloroform extraction, sodium hydroxide digestion, and ultrasonic disruption were selected as effective candidates in initial screenings, and their processing conditions were optimized. While PHA recovery by the optimized chloroform extraction was 74%, the recovered polymers contained ignorable impurities and had superior molecular properties. In contrast, sodium hydroxide digestion and ultrasonic disruption enabled PHA recovery without loss, but the purity (> 73% and ≥ 80%, respectively) and molecular properties of the recovered polymers required some improvement. Our findings suggest that these PHA recovery methods would be applicable in PHA production using WAS-derived MMC with pre- or post-processing as necessary, though they did not necessarily satisfy all the requirements in PHA production systems.</p></div>","PeriodicalId":643,"journal":{"name":"Journal of Material Cycles and Waste Management","volume":"27 5","pages":"2887 - 2898"},"PeriodicalIF":3.0000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10163-025-02242-3.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Material Cycles and Waste Management","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s10163-025-02242-3","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The production of polyhydroxyalkanoates (PHAs) using a mixed microbial culture (MMC) derived from waste activated sludge (WAS) is a promising strategy for converting wastewater treatment plants into biorefinery facilities. Despite many studies on PHA production, the recovery of PHA stored in WAS-derived MMC has received less attention. In this study, we comparatively evaluated three solvent extraction, three cellular digestion, and two mechanical disruption methods to determine their effectiveness in the recovery of PHA from WAS-derived MMC. Chloroform extraction, sodium hydroxide digestion, and ultrasonic disruption were selected as effective candidates in initial screenings, and their processing conditions were optimized. While PHA recovery by the optimized chloroform extraction was 74%, the recovered polymers contained ignorable impurities and had superior molecular properties. In contrast, sodium hydroxide digestion and ultrasonic disruption enabled PHA recovery without loss, but the purity (> 73% and ≥ 80%, respectively) and molecular properties of the recovered polymers required some improvement. Our findings suggest that these PHA recovery methods would be applicable in PHA production using WAS-derived MMC with pre- or post-processing as necessary, though they did not necessarily satisfy all the requirements in PHA production systems.

查看原文本刊更多论文

从废活性污泥中提取混合微生物培养物的聚羟基烷酸盐回收方法的比较评价

利用来自废活性污泥（WAS）的混合微生物培养（MMC）生产聚羟基烷酸酯（PHAs）是将污水处理厂转化为生物炼制设施的一种很有前途的策略。尽管有许多关于PHA生产的研究，但在was衍生的MMC中储存PHA的回收却很少受到关注。在这项研究中，我们比较评估了三种溶剂萃取、三种细胞消化和两种机械破坏方法，以确定它们在从was来源的MMC中回收PHA的有效性。初步筛选了氯仿提取、氢氧化钠消解和超声波破坏作为有效候选，并对其工艺条件进行了优化。优化后的氯仿提取法PHA的回收率为74%，回收的聚合物杂质可忽略不计，具有优良的分子性能。相比之下，氢氧化钠消解和超声波破坏可以在不损失PHA的情况下回收PHA，但回收聚合物的纯度（分别为>； 73%和≥80%）和分子性质有待提高。我们的研究结果表明，这些PHA回收方法将适用于使用was衍生的MMC生产PHA，并根据需要进行预处理或后处理，尽管它们不一定满足PHA生产系统的所有要求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Material Cycles and Waste Management 环境科学-环境科学

CiteScore

5.30

自引率

16.10%

发文量

205

审稿时长

4.8 months

期刊介绍： The Journal of Material Cycles and Waste Management has a twofold focus: research in technical, political, and environmental problems of material cycles and waste management; and information that contributes to the development of an interdisciplinary science of material cycles and waste management. Its aim is to develop solutions and prescriptions for material cycles. The journal publishes original articles, reviews, and invited papers from a wide range of disciplines related to material cycles and waste management. The journal is published in cooperation with the Japan Society of Material Cycles and Waste Management (JSMCWM) and the Korea Society of Waste Management (KSWM).