Modelling of the Systemic Inflammatory Host Response in Relation to the Microbiome of the Endodontic Infection.

IF 7.1 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
A C Georgiou, G R van der Ploeg, J A Westerhuis, S V van der Waal, A Heintz-Buschart, A K Smilde, B W Brandt
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引用次数: 0

Abstract

Aim: This study aimed to investigate systemic inflammatory responses in individuals with apical periodontitis (AP) compared to age-matched healthy controls, using advanced multiway modelling techniques. We examined associations between inflammatory mediators, age, gender, symptomatic status, and the microbial composition and function to better understand host-microbe interactions in AP.

Methods: We conducted a longitudinal study with 53 participants (27 with AP, 26 controls) aged 18 to 80. Blood inflammatory mediators were measured at multiple time points. Root canal microbiome and potential functions were analysed using 16S rRNA gene sequencing. Data were analysed using Parallel Factor Analysis (PARAFAC) and Advanced Coupled Matrix and Tensor Factorization (ACMTF) to capture time-resolved variation.

Results: PARAFAC revealed that IL-4 levels were significantly elevated in AP cases, suggesting a role for adaptive immune activation. Age strongly influenced markers such as CRP, TNF-α, and VEGF. Symptomatic AP cases showed higher CRP and lower OPG levels, indicating more active inflammation and altered bone metabolism. ACMTF identified associations between specific taxa (e.g., Parvimonas micra, Streptococcus mutans) and inflammatory mediators, with functional analysis highlighting enriched pathways like sphingolipid signalling in asymptomatic cases.

Conclusion: This study provides novel insights into the systemic immune profile of individuals with AP. IL-4 and bone metabolism markers may serve as potential biomarkers for distinguishing AP status. Our findings support the relevance of systemic inflammation in endodontic infections and underscore the value of multi-marker, multivariate approaches to better characterise disease progression and host-microbiome interactions. Such insights could contribute to improved risk stratification and personalised management in dental and systemic health contexts.

模拟与牙髓感染微生物组相关的全身炎症宿主反应。
目的:本研究旨在利用先进的多路建模技术,研究与年龄匹配的健康对照相比,根尖牙周炎(AP)患者的全身炎症反应。我们研究了炎症介质、年龄、性别、症状状态、微生物组成和功能之间的关系,以更好地了解AP中宿主-微生物的相互作用。方法:我们对53名18至80岁的参与者(27名AP患者,26名对照组)进行了纵向研究。在多个时间点测量血液炎症介质。采用16S rRNA基因测序分析根管微生物组及其潜在功能。使用并行因子分析(PARAFAC)和高级耦合矩阵和张量分解(ACMTF)对数据进行分析,以捕获时间分辨的变化。结果:PARAFAC显示IL-4水平在AP病例中显著升高,提示其具有适应性免疫激活作用。年龄强烈影响CRP、TNF-α和VEGF等标志物。有症状的AP患者表现出较高的CRP和较低的OPG水平,表明炎症更活跃,骨代谢改变。ACMTF确定了特定分类群(如微小小单胞菌、变形链球菌)与炎症介质之间的关联,功能分析突出了无症状病例中神经鞘脂信号等富集途径。结论:本研究为AP患者的全身免疫谱提供了新的见解。IL-4和骨代谢标志物可能作为区分AP状态的潜在生物标志物。我们的研究结果支持了全身性炎症与牙髓感染的相关性,并强调了多标志物、多变量方法更好地表征疾病进展和宿主-微生物组相互作用的价值。这些见解有助于改善牙齿和全身健康环境中的风险分层和个性化管理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International endodontic journal
International endodontic journal 医学-牙科与口腔外科
CiteScore
10.20
自引率
28.00%
发文量
195
审稿时长
4-8 weeks
期刊介绍: The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.
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