Comparison of PCR-Based Methods for the Detection of Canned Tuna Species

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-09-19 DOI:10.1111/1750-3841.70424

Chloe P. Castanon, Denise Hernandez, Akshay N. Khetrapal, Rosalee S. Hellberg

{"title":"Comparison of PCR-Based Methods for the Detection of Canned Tuna Species","authors":"Chloe P. Castanon, Denise Hernandez, Akshay N. Khetrapal, Rosalee S. Hellberg","doi":"10.1111/1750-3841.70424","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna products labeled as albacore, skipjack, yellowfin, and light tuna. Each sample was analyzed with CR mini-barcoding, real-time PCR, and multiplex PCR. The top-performing targeted method also underwent sensitivity testing using binary species mixtures. Real-time PCR showed the highest species identification rate, with 100% of products detected, followed by CR mini-barcoding (33%) and multiplex PCR (29%). Real-time PCR also showed excellent sensitivity, detecting 0.1%–1% of the target species in fresh and heat-treated binary species mixtures. Multiplex PCR and real-time PCR showed similar effectiveness in terms of cost and time, with a price of US$6 per sample and a total time of 3–6 h when testing all targeted species. Although CR mini-barcoding required greater costs and time, it allowed for sequencing-based detection of a range of species in the products. In conclusion, a combination of real-time PCR and CR mini-barcoding is recommended to allow for rapid screening of target species along with sequencing-based confirmation.</p>\n </section>\n \n <section>\n \n <h3> Practical Applications</h3>\n \n <p>This research provides a practical recommendation regarding the use of genetic methods for detecting species in canned tuna. Implementation of the recommended methodology is expected to enhance consumer protection and help regulatory agencies enforce accurate labeling.</p>\n </section>\n </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 9","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ift.onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70424","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Science","FirstCategoryId":"97","ListUrlMain":"https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70424","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Canned tuna is susceptible to mislabeling due to its high consumer demand, complex global supply chains, and range of prices. DNA barcoding of a short fragment of the mitochondrial control region (CR), termed the CR mini-barcode, has been established as an effective method for tuna species identification. However, the high level of DNA degradation in canned tuna products reduces the effectiveness of this method. Therefore, this study aimed to compare CR mini-barcoding to targeted (i.e., real-time or multiplex) PCR-based methods to determine the most effective approach for canned tuna species identification. DNA was extracted in duplicate from 24 canned tuna products labeled as albacore, skipjack, yellowfin, and light tuna. Each sample was analyzed with CR mini-barcoding, real-time PCR, and multiplex PCR. The top-performing targeted method also underwent sensitivity testing using binary species mixtures. Real-time PCR showed the highest species identification rate, with 100% of products detected, followed by CR mini-barcoding (33%) and multiplex PCR (29%). Real-time PCR also showed excellent sensitivity, detecting 0.1%–1% of the target species in fresh and heat-treated binary species mixtures. Multiplex PCR and real-time PCR showed similar effectiveness in terms of cost and time, with a price of US$6 per sample and a total time of 3–6 h when testing all targeted species. Although CR mini-barcoding required greater costs and time, it allowed for sequencing-based detection of a range of species in the products. In conclusion, a combination of real-time PCR and CR mini-barcoding is recommended to allow for rapid screening of target species along with sequencing-based confirmation.

Practical Applications

This research provides a practical recommendation regarding the use of genetic methods for detecting species in canned tuna. Implementation of the recommended methodology is expected to enhance consumer protection and help regulatory agencies enforce accurate labeling.

Abstract Image

查看原文本刊更多论文

基于pcr的金枪鱼罐头品种检测方法比较。

金枪鱼罐头由于其高消费需求、复杂的全球供应链和价格范围，很容易出现标签错误。对线粒体控制区（CR）短片段的DNA条形码（称为CR迷你条形码）已被确立为金枪鱼种类鉴定的有效方法。然而，金枪鱼罐头产品中DNA的高度降解降低了这种方法的有效性。因此，本研究旨在比较CR微型条形码与基于靶向（即实时或多重）pcr的方法，以确定最有效的罐装金枪鱼物种鉴定方法。从标记为长鳍金枪鱼、鲣鱼、黄鳍金枪鱼和轻金枪鱼的24种罐装金枪鱼产品中提取一式两份DNA。每个样品用CR迷你条形码、实时PCR和多重PCR进行分析。表现最好的定向方法还进行了二元混合物的敏感性测试。Real-time PCR的物种识别率最高，为100%，其次是CR mini-条形码（33%）和多重PCR（29%）。Real-time PCR也显示出良好的灵敏度，在新鲜和热处理的二元物种混合物中检测到0.1%-1%的目标物种。多重PCR和实时PCR在成本和时间方面显示出相似的有效性，每个样品的价格为6美元，检测所有目标物种的总时间为3-6小时。尽管CR迷你条形码需要更高的成本和时间，但它允许对产品中一系列物种进行基于测序的检测。总之，建议将实时PCR和CR迷你条形码相结合，以便快速筛选目标物种并进行基于测序的确认。实际应用：本研究为使用遗传方法检测金枪鱼罐头中的物种提供了实用的建议。实施建议的方法有望加强消费者保护，并帮助监管机构强制执行准确的标签。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.