Dissecting the antibacterial functions of the T6SS-2 cluster in Xanthomonas oryzae for environment and plant protection.

IF 3.7 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhi-Min Tan, Xin Zheng, Jingtong Su, Jin-Sheng Liu, Xiaoye Liang, Tong-Tong Pei, Tao Dong
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引用次数: 0

Abstract

Plant pathogens pose a serious threat to global food security, and the excessive use of chemical pesticides has raised significant ecological concerns. Harnessing microbial competition presents a promising green technology for crop protection. In this study, we examined the functions of the type VI secretion system (T6SS), a crucial microbial competition tool, in Xanthomonas oryzae pv. oryzae strain PXO99A. PXO99A exhibited significant antibacterial activities dependent on its T6SS-2 cluster. Using genome, secretome, and functional analysis, we systematically predicted T6SS-associated effectors, detected the expression of five putative effectors, and functionally validated the toxicity of a VasX_N family effector PXO_00500 and its immunity protein PXO_RS08595. We further show that PXO99A uses its T6SS-2 to kill a broad range of plant and animal pathogens in vitro and in planta. Inactivation of the T3SS functions abolished virulence but had little effect on the T6SS-2-dependent antibacterial activities. Finally, we demonstrated that the avirulent T3SS-defective mutant is effective in protecting tomatoes from Pseudomonas syringae co-infection. Collectively, these results highlight an effective biocontrol strategy for plant protection.

Importance: The growing concerns over the toxicity, environmental impact, and resistance associated with chemical pesticides underscore the urgent need for alternative pathogen management strategies. In this study, we introduce an innovative approach of "turning waste into treasure" by repurposing plant pathogens as biocontrol-like agents. By elucidating the virulence and antimicrobial functions of Xanthomonas oryzae, we demonstrate that an avirulent mutant can employ its T6SS to effectively combat a broad spectrum of human and plant pathogens. Furthermore, its ability to protect tomato plants underscores its significant potential for sustainable agricultural practices.

解析米黄单胞菌T6SS-2簇的抑菌功能及其对环境和植物保护的意义。
植物病原体对全球粮食安全构成严重威胁,化学农药的过度使用引起了严重的生态问题。利用微生物竞争是一种很有前途的作物保护绿色技术。在这项研究中,我们研究了米黄单胞菌(Xanthomonas oryzae pv) VI型分泌系统(T6SS)的功能,这是一个重要的微生物竞争工具。稻瘟病菌PXO99A。PXO99A表现出明显的抗菌活性,依赖于其T6SS-2簇。通过基因组、分泌组和功能分析,我们系统地预测了t6ss相关的效应物,检测了5种可能的效应物的表达,并从功能上验证了VasX_N家族效应物PXO_00500及其免疫蛋白PXO_RS08595的毒性。我们进一步证明PXO99A利用其T6SS-2在体外和植物体内杀死多种植物和动物病原体。失活T3SS功能可消除毒力,但对t6ss -2依赖性抗菌活性影响不大。最后,我们证明了无毒的t3ss缺陷突变体可以有效地保护番茄免受丁香假单胞菌的共同感染。总之,这些结果强调了有效的植物保护生物防治策略。重要性:人们对化学农药的毒性、环境影响和耐药性的关注日益增加,这突出表明迫切需要替代病原体管理策略。在本研究中,我们介绍了一种“变废为宝”的创新方法,即将植物病原体重新利用为生物防治类药物。通过阐明米黄单胞菌的毒力和抗菌功能,我们证明了一种无毒突变体可以利用其T6SS有效地对抗广泛的人类和植物病原体。此外,它保护番茄植物的能力凸显了它在可持续农业实践方面的巨大潜力。
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来源期刊
Applied and Environmental Microbiology
Applied and Environmental Microbiology 生物-生物工程与应用微生物
CiteScore
7.70
自引率
2.30%
发文量
730
审稿时长
1.9 months
期刊介绍: Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.
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