Long-lasting microbubble-enhanced super-resolution ultrasound imaging unveils lymphatic activity in lymph node.

IF 13.3 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-08-11 eCollection Date: 2025-01-01 DOI:10.7150/thno.117971
Jingyi Yin, Feihong Dong, Xiangui Zhang, Yuan Peng, Pengting Min, Jiabin Zhang, Shu Wang, Jing Fang, Jue Zhang
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引用次数: 0

Abstract

Rationale: As the central regulatory hub localized at the interfaces between the blood and lymphatic vascular system, lymph nodes play a pivotal role in maintaining immune homeostasis and coordinating responses to disease. Systematic evaluation of lymph node functional status holds significant clinical value. However, there are currently no established non-invasive imaging biomarkers capable of reliably evaluating lymph node activity. Methods: This study developed a novel lipid-based long-lasting microbubble contrast agent (SuperVue-MB) integrated with three-dimensional super-resolution ultrasound (SRUS) imaging to achieve multiparametric hemodynamic visualization of blood vessels within lymph nodes. Results: Experimental validation demonstrated strong correlations between SRUS-derived vascular parameters and established reference standards (r = 0.86, p < 0.01), including micro-CT angiography and histopathological markers, confirming the reliability and accuracy of the technique. Furthermore, this study introduces a new vascular subtyping strategy that systematically correlates these hemodynamic patterns with immune functional states. Specifically, the density and number of microbubble tracks associated with key vascular subtypes, exhibiting significant positive correlations with lymphatic vessel density (r = 0.91, p < 0.01). Conclusions: This study offers a promising noninvasive approach for evaluating immune activation within lymph nodes, while establishing a novel paradigm for surveillance of sentinel lymph node metastasis and immunotherapy response monitoring.

持久的微泡增强超分辨率超声成像揭示淋巴结的淋巴活动。
理论基础:作为位于血液和淋巴血管系统界面的中央调节枢纽,淋巴结在维持免疫稳态和协调对疾病的反应中起着关键作用。系统评价淋巴结功能状态具有重要的临床价值。然而,目前还没有确定的无创成像生物标志物能够可靠地评估淋巴结活动。方法:本研究开发了一种新型的脂质长效微泡造影剂(SuperVue-MB),该造影剂与三维超分辨率超声(SRUS)成像相结合,实现了淋巴结内血管的多参数血流动力学可视化。结果:实验验证表明,sru衍生血管参数与建立的参考标准(r = 0.86, p < 0.01)有很强的相关性,包括微ct血管造影和组织病理学标记,证实了该技术的可靠性和准确性。此外,本研究引入了一种新的血管分型策略,系统地将这些血流动力学模式与免疫功能状态联系起来。具体而言,微泡轨迹的密度和数量与关键血管亚型相关,与淋巴管密度呈显著正相关(r = 0.91, p < 0.01)。结论:本研究为评估淋巴结内的免疫激活提供了一种有前途的无创方法,同时为前哨淋巴结转移和免疫治疗反应监测建立了一种新的模式。
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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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