Combination of circular RNA-miRNA-mRNA expression profiles and bioinformatic analysis in ovarian endometriosis

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-09-15 DOI:10.1016/j.ygeno.2025.111112

Xiaoli Li , Ding Cui , Yang Liu

{"title":"Combination of circular RNA-miRNA-mRNA expression profiles and bioinformatic analysis in ovarian endometriosis","authors":"Xiaoli Li , Ding Cui , Yang Liu","doi":"10.1016/j.ygeno.2025.111112","DOIUrl":null,"url":null,"abstract":"<div><div>Endometriosis is a mysterious disease that affects 5 %–10 % of the women of reproductive age. Circular RNAs (circRNAs), a type of noncoding RNA, are involved in its progression, yet their regulatory mechanisms via integrated circRNA–miRNA–mRNA networks remain poorly studied. We profiled circRNAs, miRNAs, and mRNAs expression in paired eutopic and ectopic endometrium from patients with ovarian endometriosis. Differential expression analysis revealed 325 circRNAs, 295 miRNAs, and 4605 mRNAs. Using bioinformatic predictions and negative correlation analysis, we constructed a ceRNA network comprising 134 circRNAs, 72 miRNAs, and 107 mRNAs. Moreover, we identified hsa_circ_0005918/miR-504-5p/<em>PDLIM2</em> and hsa_circ_0005918/miR-105-5p/<em>OBSL1</em> as crucial regulatory axes in endometriosis. Functional experiments confirmed that hsa_circ_0005918 promotes cells migration and invasion by sponging miR-504-5p and miR-105-5p, upregulating <em>PDLIM2</em> and <em>OBSL1,</em> which may consequently contribute to the progression of endometriotic lesions. Our constructed network presents robust credibility, facilitating the exploration of complex mechanisms of circRNAs in endometriosis.</div></div>","PeriodicalId":12521,"journal":{"name":"Genomics","volume":"117 6","pages":"Article 111112"},"PeriodicalIF":3.0000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888754325001284","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Endometriosis is a mysterious disease that affects 5 %–10 % of the women of reproductive age. Circular RNAs (circRNAs), a type of noncoding RNA, are involved in its progression, yet their regulatory mechanisms via integrated circRNA–miRNA–mRNA networks remain poorly studied. We profiled circRNAs, miRNAs, and mRNAs expression in paired eutopic and ectopic endometrium from patients with ovarian endometriosis. Differential expression analysis revealed 325 circRNAs, 295 miRNAs, and 4605 mRNAs. Using bioinformatic predictions and negative correlation analysis, we constructed a ceRNA network comprising 134 circRNAs, 72 miRNAs, and 107 mRNAs. Moreover, we identified hsa_circ_0005918/miR-504-5p/PDLIM2 and hsa_circ_0005918/miR-105-5p/OBSL1 as crucial regulatory axes in endometriosis. Functional experiments confirmed that hsa_circ_0005918 promotes cells migration and invasion by sponging miR-504-5p and miR-105-5p, upregulating PDLIM2 and OBSL1, which may consequently contribute to the progression of endometriotic lesions. Our constructed network presents robust credibility, facilitating the exploration of complex mechanisms of circRNAs in endometriosis.

查看原文本刊更多论文

环状RNA-miRNA-mRNA表达谱与卵巢子宫内膜异位症生物信息学分析的结合。

子宫内膜异位症是一种神秘的疾病，影响5 %-10 %的育龄妇女。环状RNA （circRNAs）是一种非编码RNA，参与了其进展，但其通过circRNA-miRNA-mRNA集成网络的调控机制仍未得到充分研究。我们分析了来自卵巢子宫内膜异位症患者的配对异位和异位子宫内膜中环状rna、miRNAs和mrna的表达。差异表达分析显示325个circrna， 295个mirna和4605个mrna。利用生物信息学预测和负相关分析，我们构建了一个包含134个circrna、72个mirna和107个mrna的ceRNA网络。此外，我们发现hsa_circ_0005918/miR-504-5p/PDLIM2和hsa_circ_0005918/miR-105-5p/OBSL1是子宫内膜异位症的关键调控轴。功能实验证实，hsa_circ_0005918通过海绵miR-504-5p和miR-105-5p促进细胞迁移和侵袭，上调PDLIM2和OBSL1，从而可能促进子宫内膜异位症病变的进展。我们构建的网络具有强大的可信度，有助于探索环状rna在子宫内膜异位症中的复杂机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.