Molecular insights into the persistence and co-occurrence of two different carbapenem-resistant Pseudomonas aeruginosa lineages within a hospital setting.

IF 3.8 2区生物学 Q2 MICROBIOLOGY

Microbiology spectrum Pub Date : 2025-09-17 DOI:10.1128/spectrum.00433-25

Xinran Li, Kaiying Wang, Jiali Chen, Zixuan Chen, Jinhui Li, Peng Li, Xiong Liu, Suling Liu

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Abstract

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) represents a critical-priority pathogen capable of causing life-threatening, multidrug-resistant infections. We performed susceptibility testing, whole-genome sequencing, and bioinformatic analyses on 137 CRPA isolates from a Guangdong hospital. We found that the major specimen types were respiratory specimens (57/137, 41.6%) and bronchoalveolar lavage (42/137, 30.7%). All isolates were carbapenem-resistant but had low resistance to polymyxin B (0.7%, 1/137). IncP-6-positive isolates exhibited ≥2- to 32-fold higher resistance to 9/12 antibiotics (P < 0.05), with no difference to imipenem and meropenem. Fifty-four sequence types and 11 O-serogroups were identified, with ST1971 (6.6%) and O11 (29.9%) being predominant. Temporal and spatial patterns suggest persistent co-occurrence of clade 1 and clade 2 isolates, indicating potential nosocomial outbreak and clonal transmission.

Importance: The prevalence of carbapenem-resistant Pseudomonas aeruginosa (CRPA) has increased rapidly in recent years, yet few genetic and epidemiological studies on CRPA isolates have been performed. We performed susceptibility testing, whole-genome sequencing, and bioinformatic analyses on hospital isolates to investigate their resistance profiles and molecular epidemiology. These findings may offer new insights for developing effective global strategies to control CRPA and reduce untreatable infections in clinical settings.

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两种不同碳青霉烯耐药铜绿假单胞菌谱系在医院环境中的持久性和共存的分子见解。

碳青霉烯耐药铜绿假单胞菌（CRPA）是一种能够引起危及生命的多重耐药感染的关键优先病原体。我们对广东某医院的137株CRPA分离株进行了药敏试验、全基因组测序和生物信息学分析。我们发现呼吸道标本（57/137,41.6%）和支气管肺泡灌洗标本（42/137,30.7%）是主要的标本类型。所有分离株均对碳青霉烯耐药，但对多粘菌素B耐药较低（0.7%,1/137）。incp -6阳性分离株对9/12种抗生素的耐药性高2 ~ 32倍（P < 0.05），对亚胺培南和美罗培南的耐药性差异无统计学意义。共鉴定出54种序列型和11个o型血清组，以ST1971型（6.6%）和O11型（29.9%）为主。时间和空间格局提示进化支1和进化支2分离株持续共存，表明潜在的院内暴发和克隆传播。重要性：近年来耐碳青霉烯假单胞菌（Pseudomonas aeruginosa， CRPA）的流行率迅速上升，但对CRPA分离株的遗传和流行病学研究很少。我们对医院分离株进行了药敏试验、全基因组测序和生物信息学分析，以调查其耐药谱和分子流行病学。这些发现可能为制定有效的全球策略来控制CRPA和减少临床环境中无法治疗的感染提供新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology spectrum Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.20

自引率

5.40%

发文量

1800

期刊介绍： Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.