Evaluation of the Anti-inflammatory, Phagocytic, and Antioxidant Potential of ORSL® Immunity+ in Lipopolysaccharide-stimulated Human Peripheral Blood Mononuclear Cells.

Abstract

Background: Maintaining optimal hydration and electrolyte balance is essential for immune function, as it supports cellular homeostasis and immune defense mechanisms. Electrolyte-based micronutrient drinks helps replenish essential nutrients, including zinc, selenium, and vitamin E, which play key roles in modulating immune responses, mitigating oxidative stress, and regulating inflammation. Lipopolysaccharide (LPS)-induced inflammation and oxidative stress are major contributors to immune dysfunction, necessitating interventions that enhance immune resilience.

Objective: To evaluate the immunomodulatory potential of ORSL® Immunity⁺ in mitigating inflammation, enhancing phagocytic activity, and providing antioxidant protection in LPS-stimulated peripheral blood mononuclear cells (PBMCs) Materials and methods: PBMCs were isolated from human blood and stimulated with LPS to induce an inflammatory response. The effects of ORSL® Immunity+ on the immune function of treated cells were assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cytotoxicity, enzyme-linked immunosorbent assay (ELISA) for inflammatory markers [tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin 6 (IL-6), interferon gamma (IFN-γ), nitric oxide (NO)], oxygen radical absorbance capacity (ORAC) assay for antioxidant capacity, and phagocytosis assay.

Results: ORSL® Immunity+ demonstrated no cytotoxic effects on PBMCs. The treatment significantly reduced the levels of inflammatory markers, indicating an anti-inflammatory effect. Additionally, ORSL^® Immunity⁺ enhanced phagocytic activity in a dose-dependent manner against Escherichia coli, suggesting improved immune response. Antioxidant capacity was also significantly increased, as evidenced by the ORAC assay, highlighting its potential in mitigating oxidative stress.

Conclusion: ORSL® Immunity+ exhibits promising immunomodulatory properties by reducing inflammation, enhancing phagocytosis, and improving antioxidant potential in LPS-stimulated PBMCs. These findings support a potential role in immune health alongside improving hydration status, warranting further clinical evaluation.