[Investigation on the Role of Medical Recombinant Human-Derived Collagen Functional Dressings in Wound Healing].

Q4 Medicine

中国医疗器械杂志 Pub Date : 2025-07-30 DOI:10.12455/j.issn.1671-7104.250072

Xiaoxiao Gai, Xiaoxia Sun, Wenqian Ma, Zhenhua Lin, Xinyuan Li, Chenghu Liu

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引用次数: 0

Abstract

Objective: To investigate the biological effect of medical recombinant human-derived collagen functional dressings in wound healing.

Methods: MTT assay and RTCA assay were used to detect cell toxicity and proliferation. Scratch assay and Transwell cell migration assay were used to detect cell motility and migration ability. Enzyme-linked immunosorbent assay was used to detect the contents of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and platelet-endothelial cell adhesion molecule (CD31) in the supernatant of four types of cells. After animal surgery, the surgical wound was taken at 1 week, 4 weeks and 13 weeks, respectively, for hematoxylin eosin (HE) staining and immunohistochemistry to observe the inflammatory response and CD31 expression of the wound.

Results: Medical recombinant human-derived collagen functional dressing promotes cell proliferation and migration, enhances wound angiogenesis by upregulating the expression of VEGF, FGF, and CD31 in human dermal vascular endothelial cells (HDVEC) and human vascular endothelial cells (HVEC), thereby improving local blood supply to the wound, regulating the inflammatory response of the wound, and accelerating wound healing.

Conclusion: Recombinant type Ⅲ humanized collagen plays an important role in wound healing.

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医用重组人源性胶原蛋白功能敷料在伤口愈合中的作用研究

目的：探讨医用重组人源性胶原蛋白功能敷料在创面愈合中的生物学效应。方法：采用MTT法和RTCA法检测细胞毒性和增殖。采用划痕法和Transwell细胞迁移法检测细胞运动和迁移能力。采用酶联免疫吸附法检测四种细胞上清液中血管内皮生长因子（VEGF）、成纤维细胞生长因子（FGF）和血小板内皮细胞粘附分子（CD31）的含量。动物手术后，分别于1周、4周和13周取手术创面进行苏木精伊红（HE）染色和免疫组化，观察创面炎症反应和CD31表达。结果：医用重组人源性胶原功能性敷料通过上调人真皮血管内皮细胞（HDVEC）和人血管内皮细胞（HVEC）中VEGF、FGF和CD31的表达，促进细胞增殖和迁移，促进创面血管生成，从而改善创面局部血供，调节创面炎症反应，加速创面愈合。结论：重组型Ⅲ人源胶原在创面愈合中起重要作用。

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来源期刊

中国医疗器械杂志 Medicine-Medicine (all)

CiteScore

0.40

自引率

0.00%

发文量

8086

期刊介绍： Chinese Journal of Medical Instrumentation mainly reports on the development, progress, research and development, production, clinical application, management, and maintenance of medical devices and biomedical engineering. Its aim is to promote the exchange of information on medical devices and biomedical engineering in China and turn the journal into a high-quality academic journal that leads academic directions and advocates academic debates.