Exogenous acyl homoserine lactones modulate biofilm formation in Pseudomonas aeruginosa PFL-P1 and enhance phenanthrene degradation

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2025-07-16 DOI:10.1002/jctb.70023

Kumari Uma Mahto, Surajit Das

{"title":"Exogenous acyl homoserine lactones modulate biofilm formation in Pseudomonas aeruginosa PFL-P1 and enhance phenanthrene degradation","authors":"Kumari Uma Mahto, Surajit Das","doi":"10.1002/jctb.70023","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>Quorum sensing (QS) regulates several physiological behaviors in bacteria. Acyl homoserine lactones (AHLs) are self-synthesized auto-inducing molecules to form biofilm. Exogenous addition of AHLs can improve the bacterial community structure and biodegradation performance in wastewater treatment systems. However, the role of the QS-mediated interspecies interaction in phenanthrene degradation has been relatively less explored. There remains a notable research gap in understanding the effect of eavesdropping phenomena on biofilm formation, catabolic gene expression, and polycyclic aromatic hydrocarbon degradation in marine bacteria.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>Two non-cognate AHLs – <i>N</i>-hexanoyl-<span>dl</span>-homoserine lactone (C6-HSL) and <i>N</i>-3-oxo-octanoyl-<span>l</span>-homoserine lactone (3OC8-HSL) – showed affinity for LasR and RhlR QS systems in a marine bacterium, <i>Pseudomonas aeruginosa</i> PFL-P1. Exogenous supplementation of AHLs improved the specific biofilm formation (SBF) index, cell surface hydrophobicity, cell auto-aggregation, and motility. Further, the phenanthrene degradation potential of the organism increased from 85.18% to 93.82% in the presence of C6-HSL and 96.59% in the presence of 3OC8-HSL within 5 days. The biofilm-associated extracellular polymeric substances (EPS) showed high emulsification activity towards several hydrocarbons. The high binding constant (4.42 L mol<sup>−1</sup>) and binding site number (1.38), indicating binding efficiency, suggested a strong interaction between EPS and phenanthrene. The exogenous addition of AHLs upregulated the expression of <i>lasI</i>, <i>rhlI</i>, and <i>pslB</i>. The phenanthrene catabolic gene, <i>nahAc</i>, was upregulated up to 3.07-fold (<i>P</i> < 0.01) in the presence of C6-HSL and up to 4.7-fold in the presence of 3OC8-HSL (<i>P</i> < 0.0001).</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>These findings suggest that QS-mediated enhancement of pollutant biodegradation is facilitated by improved biofilm formation and catabolic gene expression. © 2025 Society of Chemical Industry (SCI).</p>\n </section>\n </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 10","pages":"2146-2162"},"PeriodicalIF":2.4000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chemical technology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jctb.70023","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND

Quorum sensing (QS) regulates several physiological behaviors in bacteria. Acyl homoserine lactones (AHLs) are self-synthesized auto-inducing molecules to form biofilm. Exogenous addition of AHLs can improve the bacterial community structure and biodegradation performance in wastewater treatment systems. However, the role of the QS-mediated interspecies interaction in phenanthrene degradation has been relatively less explored. There remains a notable research gap in understanding the effect of eavesdropping phenomena on biofilm formation, catabolic gene expression, and polycyclic aromatic hydrocarbon degradation in marine bacteria.

RESULTS

Two non-cognate AHLs – N-hexanoyl-dl-homoserine lactone (C6-HSL) and N-3-oxo-octanoyl-l-homoserine lactone (3OC8-HSL) – showed affinity for LasR and RhlR QS systems in a marine bacterium, Pseudomonas aeruginosa PFL-P1. Exogenous supplementation of AHLs improved the specific biofilm formation (SBF) index, cell surface hydrophobicity, cell auto-aggregation, and motility. Further, the phenanthrene degradation potential of the organism increased from 85.18% to 93.82% in the presence of C6-HSL and 96.59% in the presence of 3OC8-HSL within 5 days. The biofilm-associated extracellular polymeric substances (EPS) showed high emulsification activity towards several hydrocarbons. The high binding constant (4.42 L mol⁻¹) and binding site number (1.38), indicating binding efficiency, suggested a strong interaction between EPS and phenanthrene. The exogenous addition of AHLs upregulated the expression of lasI, rhlI, and pslB. The phenanthrene catabolic gene, nahAc, was upregulated up to 3.07-fold (P < 0.01) in the presence of C6-HSL and up to 4.7-fold in the presence of 3OC8-HSL (P < 0.0001).

CONCLUSION

Abstract Image

查看原文本刊更多论文

外源性酰基同丝氨酸内酯调节铜绿假单胞菌PFL-P1的生物膜形成并促进菲的降解

群体感应（Quorum sensing， QS）调节细菌的多种生理行为。酰基高丝氨酸内酯（AHLs）是一种自合成的自诱导分子，可形成生物膜。外源添加ahl可以改善废水处理系统中的细菌群落结构和生物降解性能。然而，qs介导的种间相互作用在菲降解中的作用研究相对较少。偷听现象对海洋细菌生物膜形成、分解代谢基因表达和多环芳烃降解的影响，目前还存在明显的研究空白。结果在铜绿假单胞菌PFL-P1中，两个非同源ahl - n-己醇基-dl-高丝氨酸内酯（C6-HSL）和n- 3-氧辛基-l-高丝氨酸内酯（3OC8-HSL）对LasR和rhr QS系统具有亲和力。外源补充AHLs提高了特异性生物膜形成（SBF）指数、细胞表面疏水性、细胞自聚集和运动性。在C6-HSL和3OC8-HSL的存在下，该菌的菲降解潜能在5天内分别从85.18%和96.59%提高到93.82%和93.82%。生物膜相关的胞外聚合物（EPS）对几种烃类具有较高的乳化活性。高结合常数（4.42 L mol−1）和高结合位点数（1.38）表明结合效率，表明EPS与菲之间存在强相互作用。外源添加ahl可上调lasI、rhlI和pslB的表达。在C6-HSL存在时，菲分解代谢基因nahAc上调了3.07倍（P < 0.01），在3OC8-HSL存在时上调了4.7倍（P < 0.0001）。结论通过改善生物膜的形成和分解代谢基因的表达，促进了qs介导的污染物生物降解的增强。©2025化学工业学会（SCI）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.