Efficient Expression and Purification of Recombinant Mouse Dimeric IgA

IF 3.7 3区医学 Q2 IMMUNOLOGY

European Journal of Immunology Pub Date : 2025-09-15 DOI:10.1002/eji.70055

Antonia Geisse, Tao Zhang, Jonathan Schreiber, Kristina Markova, Sophie Burkhalter, Hedda Wardemann, Andrew J. Macpherson, Tim Rollenske

引用次数: 0

Abstract

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

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重组小鼠二聚体IgA的高效表达和纯化

免疫球蛋白（Ig） A是在哺乳动物粘膜表面发现的主要抗体同型，主要以二聚体形式存在。在这里，我们提供了一种简单，可扩展，高效，广泛适用的方法，从单个B细胞Ig转录物中生产和纯化单克隆小鼠二聚体IgA，以研究单细胞水平的粘膜抗体反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Immunology 医学-免疫学

CiteScore

8.30

自引率

3.70%

发文量

224

审稿时长

2 months

期刊介绍： The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.