Airway sympathectomy attenuates inflammation, transcriptional ratios of Muc5ac and Muc5b, and airway mechanic deficits in mice delivered intranasal IL-13.

IF 3.5 2区 医学 Q1 PHYSIOLOGY
Pedro Trevizan-Baú, Amy L Fagan, Shanil P Amin, Leah R Reznikov
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引用次数: 0

Abstract

Excessive mucus in the airways is an underlying pathological feature of many airway diseases, including asthma. Therapeutic options to reduce mucus production in the airways remain limited. One possible therapeutic target is the airway sympathetic nerves. Although lung sympathetic innervation has been considered sparse, sympathetic nerves secrete neurotransmitters that act on adrenergic receptors, including β2-adrenergic receptor (β2AR). Interestingly, in experimental models, chronic use β2AR agonists can augment mucus secretion. Thus, in the present study, we tested the hypothesis that airway sympathetic nerves regulate mucus production in the airway in response to the type 2 cytokine interleukin 13 (IL-13). We performed airway sympathectomy using intranasal instillation of the synthetic neurotoxin 6-hydroxydopamine (6-OHDA). Airway sympathectomy attenuated multiple IL-13-mediated airway deficits, including density of goblet cells containing neutral mucins, transcriptional ratio of mucin 5ac (Muc5ac) to mucin 5b (Muc5b) and airway elastance and tissue damping. Although total Muc5ac and Muc5b transcript levels and Muc5ac and Muc5b protein levels in bronchoalveolar lavage were not significantly altered, these changes suggest that airway sympathectomy modifies goblet cell phenotype and mucin composition. Airway sympathectomy also dampened IL-13 mediated increases in total lung transcripts important for regulating allergic responses, including interleukin 6, complement component 3, and colony stimulating factor. This study reveals that airway sympathetic nerves regulate physiologic, molecular, and inflammatory responses to type 2 (IL-13-mediated) airway inflammation and raises the possibility that they may serve as potential targets for therapeutic intervention.

气道交感神经切除术减轻了鼻腔IL-13小鼠的炎症、Muc5ac和Muc5b的转录比率和气道力学缺陷。
气道粘液过多是包括哮喘在内的许多气道疾病的潜在病理特征。减少气道粘液产生的治疗选择仍然有限。一个可能的治疗靶点是气道交感神经。尽管肺交感神经支配被认为是稀疏的,但交感神经分泌的神经递质作用于肾上腺素能受体,包括β2-肾上腺素能受体(β2AR)。有趣的是,在实验模型中,长期使用β2AR激动剂可以增加粘液分泌。因此,在本研究中,我们验证了气道交感神经响应2型细胞因子白细胞介素13 (IL-13)调节气道粘液产生的假设。我们使用鼻内灌注合成神经毒素6-羟多巴胺(6-OHDA)进行气道交感神经切除术。气道交感神经切除术减轻了多种il -13介导的气道缺陷,包括含中性粘蛋白的杯状细胞密度、粘蛋白5ac (Muc5ac)与粘蛋白5b (Muc5b)的转录比、气道弹性和组织阻尼。尽管支气管肺泡灌洗液中Muc5ac和Muc5b转录物的总水平以及Muc5ac和Muc5b蛋白的水平没有显著改变,但这些变化表明气道交感神经切除术改变了杯状细胞的表型和粘蛋白的组成。气道交感神经切除术也抑制了IL-13介导的对调节过敏反应重要的总肺转录物的增加,包括白细胞介素6、补体成分3和集落刺激因子。本研究揭示了气道交感神经调节2型(il -13介导)气道炎症的生理、分子和炎症反应,并提出了它们可能作为治疗干预的潜在靶点的可能性。
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来源期刊
CiteScore
9.20
自引率
4.10%
发文量
146
审稿时长
2 months
期刊介绍: The American Journal of Physiology-Lung Cellular and Molecular Physiology publishes original research covering the broad scope of molecular, cellular, and integrative aspects of normal and abnormal function of cells and components of the respiratory system. Areas of interest include conducting airways, pulmonary circulation, lung endothelial and epithelial cells, the pleura, neuroendocrine and immunologic cells in the lung, neural cells involved in control of breathing, and cells of the diaphragm and thoracic muscles. The processes to be covered in the Journal include gas-exchange, metabolic control at the cellular level, intracellular signaling, gene expression, genomics, macromolecules and their turnover, cell-cell and cell-matrix interactions, cell motility, secretory mechanisms, membrane function, surfactant, matrix components, mucus and lining materials, lung defenses, macrophage function, transport of salt, water and protein, development and differentiation of the respiratory system, and response to the environment.
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