Enhancing the Quality of Single Cone Obturation Using Hydroxyapatite Precursor Grafted Nanocomplex for Dentine Conditioning: An In Vitro Study.

IF 7.1 1区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Eissa Sameer Bunashi, Mingxin Hu, Angeline Hui Cheng Lee, Chengfei Zhang, Anil Kishen, Jeffrey Wen Wei Chang
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引用次数: 0

Abstract

Aim: This study aimed to quantify the effect of chitosan-hydroxyapatite precursor nanocomplexes (C-HA) on void reduction in the root canal system and isthmus regions, characterise void distribution patterns and assess sealer-dentin interface adaptation post-conditioning.

Methodology: Vertucci type II and Yin type IV mesial root canals from 24 extracted mandibular first molars were randomly divided into two groups (n = 12): (1) C-HA conditioned and (2) control (deionised water). All canals underwent standardised preparation using ProTaper Next files with 3% NaOCl irrigation (2 mL per file change) and final irrigation with 17% EDTA (5 mL, sonic activation) followed by 3% NaOCl (5 mL) and a final deionised water rinse (5 mL). Before obturation, the C-HA group received 5 min of C-HA solution (2 mg/mL) treatment with active agitation by ProTaper Next X2 gutta-percha cone, while the control group received equivalent water treatment. Canals were obturated using the single-cone technique with Ceraseal. High-resolution Micro-CT (8.6 μm voxel size) quantified void percentages in the entire canal system and isthmus regions, with analysis stratified by coronal, middle and apical thirds. Selected specimens underwent SEM evaluation of interfacial adaptation. Statistical analysis used the independent samples t-test, two-way ANOVA with Tukey's post hoc tests (α = 0.05), and Fisher's exact test.

Results: No significant differences related to the canal/isthmus pre-treatment volumes and isthmus characteristics were noted between the two groups. Micro-CT analysis revealed significantly lower void percentages in C-HA-treated canals compared to controls (9.21% vs. 19.01% in the entire canal system and 19.07% vs. 55.65% in the isthmus regions, p < 0.001). Analysis of the void distribution patterns further demonstrated statistically significant differences between the groups in the cervical and middle root thirds (p < 0.05). SEM evaluation showed continuous, gap-free interfaces with bioactive mineralization in C-HA-treated canals, contrasting with discontinuous adaptation and sparse precipitates in controls.

Conclusion: C-HA dentine conditioning significantly improved obturation quality with Ceraseal bioceramic sealer by reducing voids in isthmus regions and enhancing sealer-dentin interfacial adaptation.

羟基磷灰石前体纳米复合物用于牙本质调节提高单锥体封闭质量的体外研究。
目的:本研究旨在量化壳聚糖-羟基磷灰石前体纳米复合物(C-HA)对根管系统和峡区空隙减少的影响,表征空隙分布模式并评估封闭物-牙本质界面适应后调节。方法:将24颗拔除的下颌第一磨牙的Vertucci II型和Yin IV型近中根管随机分为两组(n = 12):(1) C-HA条件组和(2)对照组(去离子水)。所有的管道使用ProTaper Next锉进行标准化准备,用3% NaOCl冲洗(每次锉2 mL),最后用17% EDTA (5 mL,声波激活)冲洗,然后用3% NaOCl (5 mL)和最后的去离子水冲洗(5 mL)。闭孔前,C-HA组给予C-HA溶液(2 mg/mL)处理5 min, ProTaper Next X2杜胶锥主动搅拌,对照组给予等量水处理。采用Ceraseal单锥技术封闭根管。高分辨率Micro-CT (8.6 μm体素大小)量化了整个管道系统和峡部区域的空洞百分比,并按冠状、中间和根尖三分之一进行了分层分析。选定的样品进行了界面适应性的SEM评估。统计分析采用独立样本t检验、Tukey事后检验的双向方差分析(α = 0.05)和Fisher精确检验。结果:两组治疗前管/峡部容积及峡部特征无显著差异。显微ct分析显示,C-HA处理的根管空隙率明显低于对照组(整个根管系统空隙率为9.21%比19.01%,峡部空隙率为19.07%比55.65%)。结论:C-HA牙本质调节通过减少峡部空隙和增强密封剂与牙本质界面的适应性,显著改善了Ceraseal生物陶瓷密封剂的封闭质量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International endodontic journal
International endodontic journal 医学-牙科与口腔外科
CiteScore
10.20
自引率
28.00%
发文量
195
审稿时长
4-8 weeks
期刊介绍: The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.
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