Muneeb Ur Rahman, Shumaila Arshad, Mulazim Hussain Asim, Alamgeer, Hafiz Muhammad Irfan, Nayab Tahir
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引用次数: 0
Abstract
The purpose of the study was to synthesize bergapten-loaded nanostructured lipid carriers (NLCs) to characterize and develop an HPLC method. The bergapten-loaded NCLs were characterized for size, polydispersity index (PDI), and zeta potential (ZP). The morphology of NLCs was observed by scanning electron microscopy (SEM). The drug encapsulation and loading efficiency were evaluated. Moreover, an HPLC method was developed and validated in terms of accuracy, precision, specificity, linearity, limit of detection, and limit of quantitation. Bergapten-loaded NLCs exhibited a size of 23.5 nm with 0.25 PDI, confirming narrow size distributions, whereas blank NLCs showed 20.7 nm size and 0.19 PDI. The ZP of bergapten-loaded NLCs was − 13.0 mV, which was found to be almost the same as blank NLCs. SEM images confirmed the smooth surface and spherical shape of NLCs. The HPLC-validated method for drug quantification was found to be simple and efficient. Successful fabrication of drug-loaded NLCs showed size, PDI, and ZP within range with a smooth and spherical shape, and HPLC methods showed good estimation of bergapten in NLCs.
期刊介绍：
Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
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